RRC ID 64945
著者 Yamamoto K, Hirowatari A.
タイトル Investigation of the Substrate-Binding Site of a Prostaglandin E Synthase in Bombyx mori.
ジャーナル Protein J
Abstract Prostaglandin E synthase (PGES) catalyzes the conversion of prostaglandin H2 to prostaglandin E2 in the presence of glutathione (GSH) in mammals. Amid the limited knowledge on prostaglandin and its related enzymes in insects, we recently identified PGES from the silkworm Bombyx mori (bmPGES) and determined its crystal structure complexed with GSH. In the current study, we investigated the substrate-binding site of bmPGES by site-directed mutagenesis and X-ray crystallography. We found that the residues Tyr107, Val155, Met159, and Glu203 are located in the catalytic pockets of bmPGES, and mutagenesis of each residue reduced the bmPGES activity. Our results suggest that these four residues contribute to the catalytic activity of bmPGES. Overall, this structure-function study holds implications in controlling pests by designing rational and efficient pesticides.
巻・号 40(1)
ページ 63-67
公開日 2021-2-1
DOI 10.1007/s10930-020-09956-3
PII 10.1007/s10930-020-09956-3
PMID 33403608
MeSH Amino Acid Motifs Animals Bombyx / chemistry* Bombyx / enzymology Catalytic Domain Cloning, Molecular Crystallography, X-Ray Dinitrochlorobenzene / chemistry Dinitrochlorobenzene / metabolism Dinoprostone / chemistry* Dinoprostone / metabolism Escherichia coli / genetics Escherichia coli / metabolism Gene Expression Genetic Vectors / chemistry Genetic Vectors / metabolism Glutathione / chemistry* Glutathione / metabolism Insect Proteins / chemistry* Insect Proteins / genetics Insect Proteins / metabolism Models, Molecular Mutagenesis, Site-Directed Mutation Prostaglandin-E Synthases / chemistry* Prostaglandin-E Synthases / genetics Prostaglandin-E Synthases / metabolism Protein Binding Protein Interaction Domains and Motifs Protein Structure, Secondary Recombinant Proteins / chemistry Recombinant Proteins / genetics Recombinant Proteins / metabolism Structure-Activity Relationship Substrate Specificity
IF 1.317
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