論文 - 詳細
| RRC ID | 65049 |
|---|---|
| 著者 | Miyata K, Takemoto A, Okumura S, Nishio M, Fujita N. |
| タイトル | Podoplanin enhances lung cancer cell growth in vivo by inducing platelet aggregation. |
| ジャーナル | Sci Rep |
| Abstract |
Podoplanin/Aggrus, known as a platelet aggregation-inducing factor, is frequently overexpressed in lung squamous cell carcinomas (LSCC) and glioblastomas among other tumours, and its expression has been reported to be correlated with poor prognosis. However, the contribution of podoplanin to malignant progression has been elusive. Here we demonstrate that in podoplanin-positive LSCC cells, their growth was abrogated by podoplanin knockout in vivo but not in vitro. Conversely, ectopic expression of podoplanin promoted cell growth in vivo and facilitated intratumoral platelet activation. Consistently, LSCC cells evoked podoplanin-mediated platelet aggregation (PMPA), and the releasates from platelets during PMPA promoted the growth of LSCC cells in vitro. Phospho-receptor-tyrosine-kinase array analysis revealed that epidermal growth factor receptor (EGFR) phosphorylation of LSCC cells was responsible for the growth promotion induced by platelet releasates. Treatment with an antiplatelet agent or podoplanin-neutralizing antibody depressed the growth of an LSCC tumour xenograft via suppression of EGFR phosphorylation. These results suggested that podoplanin in LSCC enhanced cell growth by inducing PMPA in vivo and contributed to malignant progression. |
| 巻・号 | 7(1) |
| ページ | 4059 |
| 公開日 | 2017-6-22 |
| DOI | 10.1038/s41598-017-04324-1 |
| PII | 10.1038/s41598-017-04324-1 |
| PMID | 28642617 |
| PMC | PMC5481446 |
| MeSH | Animals Biomarkers Blood Platelets / metabolism* Cell Line, Tumor Cell Proliferation / drug effects Disease Models, Animal ErbB Receptors / antagonists & inhibitors ErbB Receptors / metabolism Female Humans Immunohistochemistry Lung Neoplasms / blood* Lung Neoplasms / metabolism* Membrane Glycoproteins / metabolism* Membrane Glycoproteins / pharmacology Mice Platelet Aggregation* / drug effects Protein Kinase Inhibitors / pharmacology Signal Transduction / drug effects Xenograft Model Antitumor Assays |
| IF | 3.998 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | T98G(RCB1954) |