RRC ID |
650
|
Author |
Tanno K, Takeda K.
|
Title |
On the origin of six-rowed barley with brittle rachis, agriocrithon [Hordeum vulgare ssp. vulgare f. agriocrithon (Aberg) Bowd.], based on a DNA marker closely linked to the vrs1 (six-row gene) locus.
|
Journal |
Theor Appl Genet
|
Abstract |
The origin of six-rowed cultivated barley has been revealed to be more complex since the discovery of agriocrithon, a six-rowed barley with brittle rachis. The present study investigates whether such six-rowed brittle barley is wild or hybrid in nature, by analyzing genetic diversity at the cMWG699 marker locus, which is closely linked to the vrs1 (six-row gene) locus. DNA sequence analysis for 42 accessions showed only three types in six-rowed brittle barleys; in contrast, nine sequence types were found in ten wild barleys, ssp. spontaneum, in our previous study. Nucleotide diversities for the six-rowed brittle barley were 2.8-4.5 times lower than that for the ssp. spontaneum at this marker locus. The three sequence types found in the six-rowed brittle barley also appeared in the six-rowed cultivated barley. A cross-allelism test confirmed that the six-rowed character of the six-rowed brittle barley was controlled by the vrs1 locus. The nucleotide diversity and genealogy demonstrated that f. agriocrithon does not have the same level of diversity as found in wild barley, ssp. spontaneum. Consequently, f. agriocrithon does not appear to represent genuinely wild populations, but more probably originated from hybridization between ssp. spontaneum and six-rowed cultivated barley.
|
Volume |
110(1)
|
Pages |
145-50
|
Published |
2004-12-1
|
DOI |
10.1007/s00122-004-1816-4
|
PMID |
15526085
|
MeSH |
Alleles
Base Sequence
Crosses, Genetic
DNA, Plant / genetics
Genes, Plant*
Genetic Linkage
Genetic Markers
Hordeum / anatomy & histology*
Hordeum / classification
Hordeum / genetics*
Phenotype
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Polymorphism, Single Nucleotide
Sequence Homology, Nucleic Acid
|
IF |
4.439
|
Times Cited |
18
|
WOS Category
|
AGRONOMY
HORTICULTURE
PLANT SCIENCES
GENETICS & HEREDITY
|
Resource |
Barley |
OUH641
OUH662
OUH663
OUH765
OUH768
OUH785
OUH786
OUH787
OUH788
OUH789 etc. |