論文 - 詳細
| RRC ID | 65131 |
|---|---|
| 著者 | Komatsu W, Itoh K, Akutsu S, Kishi H, Ohhira S. |
| タイトル | Nasunin inhibits the lipopolysaccharide-induced pro-inflammatory mediator production in RAW264 mouse macrophages by suppressing ROS-mediated activation of PI3 K/Akt/NF-κB and p38 signaling pathways. |
| ジャーナル | Biosci Biotechnol Biochem |
| Abstract |
Nasunin is a major anthocyanin in eggplant peel. The purpose of this study was to examine the anti-inflammatory effects of nasunin in lipopolysaccharide (LPS)-stimulated RAW264 macrophages and to identify the molecular mechanisms underlying these effects. We found that nasunin reduced the LPS-induced secretion of tumor necrosis factor-α, interleukin-6 and nitric oxide, and expression of inducible nitric oxide synthase in a dose-dependent manner. Nasunin diminished LPS-induced nuclear factor-κB (NF-κB) activation by suppressing the degradation of inhibitor of κB-α and nuclear translocation of p65 subunit of NF-κB. Nasunin also attenuated the phosphorylation of Akt and p38, signaling molecules involved in pro-inflammatory mediator production. Moreover, nasunin inhibited the intracellular accumulation of ROS, leading to the suppression of NF-κB activation, Akt and p38 phosphorylation, and subsequent pro-inflammatory mediator production. These findings suggest that nasunin exerts an anti-inflammatory effect and this effect is mediated, at least in part, by its antioxidant activity. |
| 巻・号 | 81(10) |
| ページ | 1956-1966 |
| 公開日 | 2017-10-1 |
| DOI | 10.1080/09168451.2017.1362973 |
| PMID | 28812425 |
| MeSH | Animals Anthocyanins / pharmacology* Enzyme Activation / drug effects Gene Expression Regulation, Enzymologic / drug effects Inflammation Mediators / metabolism* Lipopolysaccharides / antagonists & inhibitors Lipopolysaccharides / pharmacology* MAP Kinase Signaling System / drug effects* Macrophages / cytology Macrophages / drug effects* Macrophages / metabolism Mice NF-kappa B / metabolism Nitric Oxide / biosynthesis Nitric Oxide Synthase Type II / metabolism Phosphatidylinositol 3-Kinases / metabolism Proto-Oncogene Proteins c-akt / metabolism RAW 264.7 Cells Reactive Oxygen Species / metabolism* p38 Mitogen-Activated Protein Kinases / metabolism |
| IF | 1.516 |
| リソース情報 | |
| ヒト・動物細胞 | RAW 264(RCB0535) |