RRC ID 65166
Author Bill A, Gutierrez A, Kulkarni S, Kemp C, Bonenfant D, Voshol H, Duvvuri U, Gaither LA.
Title ANO1/TMEM16A interacts with EGFR and correlates with sensitivity to EGFR-targeting therapy in head and neck cancer.
Journal Oncotarget
Abstract The epidermal growth factor receptor (EGFR) contributes to the pathogenesis of head&neck squamous cell carcinoma (HNSCC). However, only a subset of HNSCC patients benefit from anti-EGFR targeted therapy. By performing an unbiased proteomics screen, we found that the calcium-activated chloride channel ANO1 interacts with EGFR and facilitates EGFR-signaling in HNSCC. Using structural mutants of EGFR and ANO1 we identified the trans/juxtamembrane domain of EGFR to be critical for the interaction with ANO1. Our results show that ANO1 and EGFR form a functional complex that jointly regulates HNSCC cell proliferation. Expression of ANO1 affected EGFR stability, while EGFR-signaling elevated ANO1 protein levels, establishing a functional and regulatory link between ANO1 and EGFR. Co-inhibition of EGFR and ANO1 had an additive effect on HNSCC cell proliferation, suggesting that co-targeting of ANO1 and EGFR could enhance the clinical potential of EGFR-targeted therapy in HNSCC and might circumvent the development of resistance to single agent therapy. HNSCC cell lines with amplification and high expression of ANO1 showed enhanced sensitivity to Gefitinib, suggesting ANO1 overexpression as a predictive marker for the response to EGFR-targeting agents in HNSCC therapy. Taken together, our results introduce ANO1 as a promising target and/or biomarker for EGFR-directed therapy in HNSCC.
Volume 6(11)
Pages 9173-88
Published 2015-4-20
DOI 10.18632/oncotarget.3277
PII 3277
PMID 25823819
PMC PMC4496210
MeSH Anoctamin-1 Carcinoma, Squamous Cell / pathology* Cell Line, Tumor Cell Proliferation Chloride Channels / genetics Chloride Channels / metabolism* ErbB Receptors / antagonists & inhibitors ErbB Receptors / genetics ErbB Receptors / metabolism* Fusion Regulatory Protein 1, Heavy Chain / genetics Gefitinib HEK293 Cells Head and Neck Neoplasms / pathology* Humans Multiprotein Complexes / metabolism Mutation / genetics Neoplasm Proteins / genetics Neoplasm Proteins / metabolism* Protein Kinase Inhibitors / pharmacology* Protein Structure, Tertiary / genetics Quinazolines / pharmacology* RNA Interference RNA, Small Interfering Signal Transduction Squamous Cell Carcinoma of Head and Neck
IF 5.168
Human and Animal Cells 293T(RCB2202) TE-1(RCB1894) TE-11(RCB2100) TE-14(RCB2101) TE-15(RCB1951)