論文 - 詳細
| RRC ID | 65281 |
|---|---|
| 著者 | Raduwan H, Sasidharan S, Burgos LC, Wallace AG, Soto MC. |
| タイトル | RhoGAP RGA-8 supports morphogenesis in C. elegans by polarizing epithelia. |
| ジャーナル | Biol Open |
| Abstract |
CDC-42 regulation of non-muscle myosin/NMY-2 is required for polarity maintenance in the one-cell embryo of Caenorhabditis elegans CDC-42 and NMY-2 regulate polarity throughout embryogenesis, but their contribution to later events of morphogenesis are less understood. We have shown that epidermal enclosure requires the GTPase CED-10/Rac1 and WAVE/Scar complex, its effector, to promote protrusions that drive enclosure through the branch actin regulator Arp2/3. Our analysis here of RGA-8, a homolog of SH3BP1/Rich1/ARHGAP17/Nadrin, with BAR and RhoGAP motifs, suggests it regulates CDC-42, so that actin and myosin/NMY-2 promote ventral enclosure during embryonic morphogenesis. Genetic and molecular data suggest RGA-8 regulates CDC-42, and phenocopies the CDC-42 pathway regulators WASP-1/WSP-1 and the F-BAR proteins TOCA-1 and TOCA-2. Live imaging shows RGA-8 and WSP-1 enrich myosin and regulate F-actin in migrating epidermal cells during ventral enclosure. Loss of RGA-8 alters membrane recruitment of active CDC-42. We propose TOCA proteins and RGA-8 use BAR domains to localize and regenerate CDC-42 activity, thus regulating F-actin levels, through the branched actin regulator WSP-1, and myosin enrichment. RhoGAP RGA-8 thus polarizes epithelia, to promote cell migrations and cell shape changes of embryonic morphogenesis. |
| 巻・号 | 9(11) |
| 公開日 | 2020-11-26 |
| DOI | 10.1242/bio.056911 |
| PII | 9/11/bio056911 |
| PMID | 33243762 |
| PMC | PMC7710025 |
| MeSH | Animals Caenorhabditis elegans / embryology Caenorhabditis elegans Proteins / metabolism Cell Cycle Proteins / metabolism Epithelium / enzymology* Epithelium / metabolism* GTP-Binding Proteins / metabolism GTPase-Activating Proteins / metabolism* Morphogenesis / genetics* Organogenesis* / genetics Signal Transduction |
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オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm2056 |