| RRC ID |
65303
|
| Author |
Ohnishi K, Saito S, Miura T, Ohta A, Tominaga M, Sokabe T, Kuhara A.
|
| Title |
OSM-9 and OCR-2 TRPV channels are accessorial warm receptors in Caenorhabditis elegans temperature acclimatisation.
|
| Journal |
Sci Rep
|
| Abstract |
Caenorhabditis elegans (C. elegans) exhibits cold tolerance and temperature acclimatisation regulated by a small number of head sensory neurons, such as the ADL temperature-sensing neurons that express three transient receptor potential vanilloid (TRPV) channel subunits, OSM-9, OCR-2, and OCR-1. Here, we show that an OSM-9/OCR-2 regulates temperature acclimatisation and acts as an accessorial warmth-sensing receptor in ADL neurons. Caenorhabditis elegans TRPV channel mutants showed abnormal temperature acclimatisation. Ectopic expression of OSM-9 and OCR-2 in non-warming-responsive gustatory neurons in C. elegans and Xenopus oocytes revealed that OSM-9 and OCR-2 cooperatively responded to warming; however, neither TRPV subunit alone was responsive to warming. A warming-induced OSM-9/OCR-2-mediated current was detectable in Xenopus oocytes, yet ADL in osm-9 ocr-2 double mutant responds to warming; therefore, an OSM-9/OCR-2 TRPV channel and as yet unidentified temperature receptor might coordinate transmission of temperature signalling in ADL temperature-sensing neurons. This study demonstrates direct sensation of warming by TRPV channels in C. elegans.
|
| Volume |
10(1)
|
| Pages |
18566
|
| Published |
2020-10-29
|
| DOI |
10.1038/s41598-020-75302-3
|
| PII |
10.1038/s41598-020-75302-3
|
| PMID |
33122746
|
| PMC |
PMC7596061
|
| MeSH |
Acclimatization / genetics*
Animals
Caenorhabditis elegans / genetics*
Caenorhabditis elegans Proteins / genetics*
Mutation / genetics
Nerve Tissue Proteins / genetics*
Oocytes / physiology
Sensation / genetics
Sensory Receptor Cells / physiology
Signal Transduction / genetics
TRPV Cation Channels / genetics*
Temperature
Xenopus / genetics
|
| Resource |
| C.elegans |
tm6403 |
