RRC ID 65303
Author Ohnishi K, Saito S, Miura T, Ohta A, Tominaga M, Sokabe T, Kuhara A.
Title OSM-9 and OCR-2 TRPV channels are accessorial warm receptors in Caenorhabditis elegans temperature acclimatisation.
Journal Sci Rep
Abstract Caenorhabditis elegans (C. elegans) exhibits cold tolerance and temperature acclimatisation regulated by a small number of head sensory neurons, such as the ADL temperature-sensing neurons that express three transient receptor potential vanilloid (TRPV) channel subunits, OSM-9, OCR-2, and OCR-1. Here, we show that an OSM-9/OCR-2 regulates temperature acclimatisation and acts as an accessorial warmth-sensing receptor in ADL neurons. Caenorhabditis elegans TRPV channel mutants showed abnormal temperature acclimatisation. Ectopic expression of OSM-9 and OCR-2 in non-warming-responsive gustatory neurons in C. elegans and Xenopus oocytes revealed that OSM-9 and OCR-2 cooperatively responded to warming; however, neither TRPV subunit alone was responsive to warming. A warming-induced OSM-9/OCR-2-mediated current was detectable in Xenopus oocytes, yet ADL in osm-9 ocr-2 double mutant responds to warming; therefore, an OSM-9/OCR-2 TRPV channel and as yet unidentified temperature receptor might coordinate transmission of temperature signalling in ADL temperature-sensing neurons. This study demonstrates direct sensation of warming by TRPV channels in C. elegans.
Volume 10(1)
Pages 18566
Published 2020-10-29
DOI 10.1038/s41598-020-75302-3
PII 10.1038/s41598-020-75302-3
PMID 33122746
PMC PMC7596061
MeSH Acclimatization / genetics* Animals Caenorhabditis elegans / genetics* Caenorhabditis elegans Proteins / genetics* Mutation / genetics Nerve Tissue Proteins / genetics* Oocytes / physiology Sensation / genetics Sensory Receptor Cells / physiology Signal Transduction / genetics TRPV Cation Channels / genetics* Temperature Xenopus / genetics
C.elegans tm6403