RRC ID 65329
Author Wang Y, Weng C, Chen X, Zhou X, Huang X, Yan Y, Zhu C.
Title CDE-1 suppresses the production of risiRNA by coupling polyuridylation and degradation of rRNA.
Journal BMC Biol
Abstract BACKGROUND:Modification of RNAs, particularly at the terminals, is critical for various essential cell processes; for example, uridylation is implicated in tumorigenesis, proliferation, stem cell maintenance, and immune defense against viruses and retrotransposons. Ribosomal RNAs can be regulated by antisense ribosomal siRNAs (risiRNAs), which downregulate pre-rRNAs through the nuclear RNAi pathway in Caenorhabditis elegans. However, the biogenesis and regulation of risiRNAs remain obscure. Previously, we showed that 26S rRNAs are uridylated at the 3'-ends by an unknown terminal polyuridylation polymerase before the rRNAs are degraded by a 3' to 5' exoribonuclease SUSI-1(ceDIS3L2).
RESULTS:Here, we found that CDE-1, one of the three C.elegans polyuridylation polymerases (PUPs), is specifically involved in suppressing risiRNA production. CDE-1 localizes to perinuclear granules in the germline and uridylates Argonaute-associated 22G-RNAs, 26S, and 5.8S rRNAs at the 3'-ends. Immunoprecipitation followed by mass spectrometry (IP-MS) revealed that CDE-1 interacts with SUSI-1(ceDIS3L2). Consistent with these results, both CDE-1 and SUSI-1(ceDIS3L2) are required for the inheritance of RNAi.
CONCLUSIONS:This work identified a rRNA surveillance machinery of rRNAs that couples terminal polyuridylation and degradation.
Volume 18(1)
Pages 115
Published 2020-9-4
DOI 10.1186/s12915-020-00850-z
PII 10.1186/s12915-020-00850-z
PMID 32887607
PMC PMC7472701
MeSH Animals Caenorhabditis elegans / genetics* Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / metabolism Cell Cycle Proteins / genetics* Cell Cycle Proteins / metabolism Gene Silencing Germ Cells / metabolism RNA Interference RNA, Helminth / metabolism* RNA, Ribosomal / metabolism* RNA, Small Interfering / metabolism* Uridine / metabolism*
C.elegans tm936 tm1021 tm4344 tm5089 tm1200