論文 - 詳細
| RRC ID | 65352 |
|---|---|
| 著者 | Hefel A, Honda M, Cronin N, Harrell K, Patel P, Spies M, Smolikove S. |
| タイトル | RPA complexes in Caenorhabditis elegans meiosis; unique roles in replication, meiotic recombination and apoptosis. |
| ジャーナル | Nucleic Acids Res |
| Abstract |
Replication Protein A (RPA) is a critical complex that acts in replication and promotes homologous recombination by allowing recombinase recruitment to processed DSB ends. Most organisms possess three RPA subunits (RPA1, RPA2, RPA3) that form a trimeric complex critical for viability. The Caenorhabditis elegans genome encodes RPA-1, RPA-2 and an RPA-2 paralog RPA-4. In our analysis, we determined that RPA-2 is critical for germline replication and normal repair of meiotic DSBs. Interestingly, RPA-1 but not RPA-2 is essential for somatic replication, in contrast to other organisms that require both subunits. Six different hetero- and homodimeric complexes containing permutations of RPA-1, RPA-2 and RPA-4 can be detected in whole animal extracts. Our in vivo studies indicate that RPA-1/4 dimer is less abundant in the nucleus and its formation is inhibited by RPA-2. While RPA-4 does not participate in replication or recombination, we find that RPA-4 inhibits RAD-51 filament formation and promotes apoptosis of a subset of damaged nuclei. Altogether these findings point to sub-functionalization and antagonistic roles of RPA complexes in C. elegans. |
| 巻・号 | 49(4) |
| ページ | 2005-2026 |
| 公開日 | 2021-2-26 |
| DOI | 10.1093/nar/gkaa1293 |
| PII | 6105279 |
| PMID | 33476370 |
| PMC | PMC7913698 |
| MeSH | Animals Apoptosis* Caenorhabditis elegans / genetics* Caenorhabditis elegans Proteins / analysis Caenorhabditis elegans Proteins / metabolism Caenorhabditis elegans Proteins / physiology* DNA Breaks, Double-Stranded DNA Replication* Meiosis / genetics* Mitosis / genetics Rad51 Recombinase / analysis Recombination, Genetic* Replication Protein A / metabolism Replication Protein A / physiology* |
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オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 10 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm2026 tm1524 tm202 |