RRC ID 65427
Author Filho GS, Caballé-Serrano J, Sawada K, Bosshardt DD, Bianchini MA, Buser D, Gruber R.
Title Conditioned medium of demineralized freeze-dried bone activates gene expression in periodontal fibroblasts in vitro.
Journal J Periodontol
Abstract BACKGROUND:Demineralized bone matrix (DBM) is used for the treatment of osseous defects. Conditioned medium from native bone chips can activate transforming growth factor (TGF)-β signaling in mesenchymal cells. The aim of this study is to determine whether processing of native bone into DBM affects the activity of the conditioned medium.
METHODS:Porcine cortical bone blocks were subjected to defatting, different concentrations of hydrochloric acid, and various temperatures. DBM was lyophilized, ground, and placed into culture medium. Human gingiva and periodontal fibroblasts were exposed to the respective conditioned medium obtained from DBM (DBCM). Changes in the expression of TGF-β target genes were determined.
RESULTS:DBCM altered the expression of TGF-β target genes (e.g., adrenomedullin, pentraxin 3, KN motif and ankyrin repeat domains 4, interleukin 11, NADPH oxidase 4, and BTB [POZ] domain containing 11) by at least five-fold. The response was observed in fibroblasts from both sources. Defatting lowered the activity of DBCM. The TGF-β receptor type I kinase inhibitor SB431542 [4-(4-(benzo[d][1,3]dioxol-5-yl)-5-(pyridin-2-yl)-1H-imidazol-2-yl)benzamide] but not the inhibitor of bone morphogenetic protein receptor dorsomorphin, blocked the effects of DBCM on gene expression. Moreover, conditioned medium obtained from commercial human DBM modulated the expression of TGF-β target genes.
CONCLUSION:The findings suggest that the DBCM can activate TGF-β signaling in oral fibroblasts.
Volume 86(6)
Pages 827-34
Published 2015-6-1
DOI 10.1902/jop.2015.140676
PMID 25786563
MeSH AMP-Activated Protein Kinases / antagonists & inhibitors Acute-Phase Proteins / analysis Adrenomedullin / analysis Animals Ankyrin Repeat / genetics Benzamides / pharmacology Bone Demineralization Technique Bone Matrix / drug effects Bone Matrix / physiology* C-Reactive Protein / analysis Cell Line Cells, Cultured Culture Media, Conditioned Dioxoles / pharmacology Fibroblasts / metabolism Freeze Drying Gingiva / cytology Gingiva / metabolism* Humans Hydrochloric Acid / chemistry Interleukin-11 / analysis Mesenchymal Stem Cells / metabolism Mice NADPH Oxidase 4 NADPH Oxidases / analysis Nuclear Proteins / analysis Periodontium / cytology Periodontium / metabolism* Pyrazoles / pharmacology Pyrimidines / pharmacology Receptors, Transforming Growth Factor beta / antagonists & inhibitors Serum Amyloid P-Component / analysis Signal Transduction / drug effects Signal Transduction / physiology Swine Tissue Preservation / methods* Transforming Growth Factor beta / drug effects Transforming Growth Factor beta / metabolism*
IF 3.742
Human and Animal Cells ST2(RCB0224)