Reference - Detail
| RRC ID | 65509 |
|---|---|
| Author | Miki T, Nakai T, Hashimoto M, Kajiwara K, Tsutsumi H, Mihara H. |
| Title | Intracellular artificial supramolecules based on de novo designed Y15 peptides. |
| Journal | Nat Commun |
| Abstract |
De novo designed self-assembling peptides (SAPs) are promising building blocks of supramolecular biomaterials, which can fulfill a wide range of applications, such as scaffolds for tissue culture, three-dimensional cell culture, and vaccine adjuvants. Nevertheless, the use of SAPs in intracellular spaces has mostly been unexplored. Here, we report a self-assembling peptide, Y15 (YEYKYEYKYEYKYEY), which readily forms β-sheet structures to facilitate bottom-up synthesis of functional protein assemblies in living cells. Superfolder green fluorescent protein (sfGFP) fused to Y15 assembles into fibrils and is observed as fluorescent puncta in mammalian cells. Y15 self-assembly is validated by fluorescence anisotropy and pull-down assays. By using the Y15 platform, we demonstrate intracellular reconstitution of Nck assembly, a Src-homology 2 and 3 domain-containing adaptor protein. The artificial clusters of Nck induce N-WASP (neural Wiskott-Aldrich syndrome protein)-mediated actin polymerization, and the functional importance of Nck domain valency and density is evaluated. |
| Volume | 12(1) |
| Pages | 3412 |
| Published | 2021-6-7 |
| DOI | 10.1038/s41467-021-23794-6 |
| PII | 10.1038/s41467-021-23794-6 |
| PMID | 34099696 |
| PMC | PMC8185068 |
| MeSH | Actins / metabolism Adaptor Proteins, Signal Transducing / metabolism* Animals Biocompatible Materials / chemistry Biocompatible Materials / metabolism* COS Cells Chlorocebus aethiops HEK293 Cells Humans Oncogene Proteins / metabolism* Peptide Biosynthesis, Nucleic Acid-Independent Peptides / chemistry Peptides / metabolism* Protein Conformation, beta-Strand Protein Domains Protein Multimerization Wiskott-Aldrich Syndrome Protein, Neuronal / metabolism |
| IF | 12.121 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 25 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | 293(RCB1637) COS-7(RCB0539) |