論文 - 詳細
| RRC ID | 65588 |
|---|---|
| 著者 | Shimizu N, Kawakami K, Ishitani T. |
| タイトル | Visualization and exploration of Tcf/Lef function using a highly responsive Wnt/β-catenin signaling-reporter transgenic zebrafish. |
| ジャーナル | Dev Biol |
| Abstract |
Evolutionarily conserved Tcf/Lef transcription factors (Lef1, Tcf7, Tcf7l1, and Tcf7l2) mediate gene expression regulated by Wnt/β-catenin signaling, which has multiple roles in early embryogenesis, organogenesis, adult tissue homeostasis, and tissue regeneration. However, the spatiotemporal dynamics of Tcf/Lef activity during these events remain poorly understood. We generated stable transgenic zebrafish lines carrying a new Wnt/β-catenin signaling reporter, Tcf/Lef-miniP:dGFP. The reporter revealed the transcriptional activities of four Tcf/Lef members controlled by Wnt/β-catenin signaling, which were expressed in known Wnt/β-catenin signaling-active sites during embryogenesis, organ development and growth, and tissue regeneration. We used the transgenic lines to demonstrate the contribution of Tcf/Lef-mediated Wnt/β-catenin signaling to the development of the anterior lateral line, dorsal and secondary posterior lateral lines, and gill filaments. Thus, these reporter lines are highly useful tools for studying Tcf/Lef-mediated Wnt/β-catenin signaling-dependent processes. |
| 巻・号 | 370(1) |
| ページ | 71-85 |
| 公開日 | 2012-10-1 |
| DOI | 10.1016/j.ydbio.2012.07.016 |
| PII | S0012-1606(12)00399-5 |
| PMID | 22842099 |
| MeSH | Animals Animals, Genetically Modified / embryology* Blotting, Southern Cell Culture Techniques DNA Primers / genetics Gene Expression Regulation, Developmental / genetics Gene Expression Regulation, Developmental / physiology* Genes, Reporter / genetics Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism HEK293 Cells Humans In Situ Hybridization Lateral Line System / embryology* Plasmids / genetics Polymerase Chain Reaction Signal Transduction / genetics Signal Transduction / physiology* TCF Transcription Factors / genetics TCF Transcription Factors / physiology* Wnt Proteins / genetics Wnt Proteins / metabolism Zebrafish / embryology* beta Catenin / genetics beta Catenin / metabolism |
| IF | 2.896 |
| リソース情報 | |
| ゼブラフィッシュ | Tg(6xTcf/LefBS-miniP:d2EGFP) |