論文 - 詳細
| RRC ID | 65935 |
|---|---|
| 著者 | Tseng JH, Bisogna M, Hoang LN, Olvera N, Rodriguez-Aguayo C, Lopez-Berestein G, Sood AK, Levine DA, Jelinic P. |
| タイトル | miR-200c-driven Mesenchymal-To-Epithelial Transition is a Therapeutic Target in Uterine Carcinosarcomas. |
| ジャーナル | Sci Rep |
| Abstract |
Uterine carcinosarcomas (UCSs) are highly aggressive malignancies associated with poor prognoses and limited treatment options. These tumors are hypothesized to develop from the endometrial adenocarcinoma (EAC) through epithelial-mesenchymal transition (EMT). We test this long-standing hypothesis by depleting miR-200, a family of microRNAs critical for EMT, in EAC cell lines. Our data suggest that UCSs do not develop from EACs via EMT. Clinically more relevant, we show that miR-200 expression in UCS cells induces a robust mesenchymal-epithelial transition (MET). Using in vitro and murine xenograft models, we demonstrate decreased growth and aggressiveness of miR-200-overexpressing UCS cell lines. Whole transcriptome analysis confirmed changes consistent with an MET and also revealed changes in angiogenic genes expression. Finally, by treatment of UCS-xenografted mice with miR-200c incorporated in DOPC nanoliposomes, we demonstrate anti-tumor activities. These findings suggest that ectopic miR-200 expression using advanced microRNA therapeutics may be a potential treatment approach for patients with UCS. |
| 巻・号 | 7(1) |
| ページ | 3614 |
| 公開日 | 2017-6-15 |
| DOI | 10.1038/s41598-017-03972-7 |
| PII | 10.1038/s41598-017-03972-7 |
| PMID | 28620240 |
| PMC | PMC5472620 |
| MeSH | Animals Carcinosarcoma / genetics* Carcinosarcoma / pathology* Cell Adhesion Cell Movement Cell Proliferation Cell Survival Computational Biology / methods Disease Models, Animal Epithelial-Mesenchymal Transition / genetics* Female Gene Expression Profiling Gene Expression Regulation, Neoplastic Humans Mice MicroRNAs / genetics* Mutation Neovascularization, Pathologic / genetics Uterine Neoplasms / genetics* Uterine Neoplasms / pathology* Xenograft Model Antitumor Assays |
| IF | 3.998 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | JHUCS-1(RCB1547) |