論文 - 詳細
| RRC ID | 66322 |
|---|---|
| 著者 | Watanabe Y, Watanabe S, Fukui Y, Nishiwaki H. |
| タイトル | Functional and structural characterization of a novel L-fucose mutarotase involved in non-phosphorylative pathway of L-fucose metabolism. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
Mutarotases catalyze the α-β anomeric conversion of monosaccharide, and play a key role in utilizing sugar as enzymes involved in sugar metabolism have specificity for the α- or β-anomer. In spite of the sequential similarity to l-rhamnose mutarotase protein superfamily (COG3254: RhaM), the ACAV_RS08160 gene in Acidovorax avenae ATCC 19860 (AaFucM) is located in a gene cluster related to non-phosphorylative l-fucose and l-galactose metabolism, and transcriptionally induced by these carbon sources; therefore, the physiological role remains unclear. Here, we report that AaFucM possesses mutarotation activity only toward l-fucose by saturation difference (SD) NMR experiments. Moreover, we determined the crystal structures of AaFucM in the apo form and in the l-fucose-bound form at resolutions of 2.21 and 1.75 Å, respectively. The overall structural folding was clearly similar to the RhaM members, differed from the known l-fucose mutarotase (COG4154: FucU), strongly indicating their convergent evolution. The structure-based mutational analyses suggest that Tyr18 is important for catalytic action, and that Gln87 and Trp99 are involved in the l-fucose-specific recognition. |
| 巻・号 | 528(1) |
| ページ | 21-27 |
| 公開日 | 2020-7-12 |
| DOI | 10.1016/j.bbrc.2020.05.094 |
| PII | S0006-291X(20)31015-9 |
| PMID | 32448506 |
| MeSH | Calorimetry Carbohydrate Epimerases / chemistry* Carbohydrate Epimerases / metabolism* Catalytic Domain Comamonadaceae / enzymology Crystallography, X-Ray DNA Mutational Analysis Evolution, Molecular Fucose / chemistry Fucose / metabolism* Magnetic Resonance Spectroscopy Models, Molecular Phosphorylation Structure-Activity Relationship |
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
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| リソース情報 | |
| 一般微生物 | JCM20985 |