論文 - 詳細
| RRC ID | 66610 |
|---|---|
| 著者 | Li T, Zhuo Y, Jin CZ, Wu X, Ko SR, Jin FJ, Ahn CY, Oh HM, Lee HG, Jin L. |
| タイトル | Genomic insights into a novel species Rhodoferax aquaticus sp. nov., isolated from freshwater. |
| ジャーナル | Int J Syst Evol Microbiol |
| Abstract |
A novel non-phototrophic member of the genus Rhodoferax was obtained from freshwater. The purpose of this study was to analyse the genome of a nonphototrophic strain and propose a new species based on its phylogenetic, genomic, physiological and chemotaxonomic characteristics. The results of phylogenetic analysis based on 16S rRNA gene sequences supports that the strain, designated Gr-4T, has a close relationship to the genus Rhodoferax. The observed average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain Gr-4T and its closest related strains were 72.3-74.6 % and 21.9-22.8 %, respectively. These values were much lower than the species separation thresholds for ANI or dDDH of 95-96 and 70 %, respectively, and in fact fall in the intergeneric range. Strain Gr-4T does not contain RuBisCO-related genes, but does contain GS/GOGAT pathway-related genes enabling nitrate ammonification. A polyphasic study and a genomic-level investigation were done to establish the taxonomic status of strain Gr-4T. Based on the phylogenetic, genomic and physiological differences, it is proposed that the isolate be classified to the genus Rhodoferax as Rhodoferax aquaticus sp. nov. with isolate Gr-4T (=KCTC 32394T=JCM 19166T) as the type strain. |
| 巻・号 | 70(8) |
| ページ | 4653-4660 |
| 公開日 | 2020-8-1 |
| DOI | 10.1099/ijsem.0.004325 |
| PMID | 32672530 |
| MeSH | Bacterial Typing Techniques Base Composition Comamonadaceae / classification* Comamonadaceae / isolation & purification DNA, Bacterial / genetics Fatty Acids / chemistry Fresh Water / microbiology* Genes, Bacterial Nucleic Acid Hybridization Phylogeny* RNA, Ribosomal, 16S / genetics Republic of Korea Sequence Analysis, DNA |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Blogs |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM19166 |