論文 - 詳細
| RRC ID | 66625 |
|---|---|
| 著者 | He SW, Wang X, Guo HB, Han JG, Thin KK, Gao JS, Ma LA, Zhang XX. |
| タイトル | Gracilibacillus oryzae sp. nov., isolated from rice seeds. |
| ジャーナル | Int J Syst Evol Microbiol |
| Abstract |
A Gram-stain-positive, facultatively anaerobic, endospore-forming bacterium, designated strain TD8T, was isolated from surface-sterilized rice seeds (Oryza sativa L.). Phylogenetic analysis of the 16S rRNA gene indicated that strain TD8T should be placed within the genus Gracilibacillus (95.2-99.0 % sequence similarity); it exhibited highest similarities to Gracilibacillus ureilyticus CGMCC 1.7727T (99.0 %), 'Gracilibacillus xinjiangensis' CGMCC 1.12449T (98.9 %) and Gracilibacillus dipsosauri CGMCC 1.3642T (97.5 %). Chemotaxonomic analysis showed that menaquinone-7 (MK-7) was the major isoprenoid quinone. Diphosphatidylglycerol, phosphatidylglycerol and one unidentified phospholipid were the major cellular polar lipids, and the major fatty acids were anteiso-C15 : 0, anteiso-C17 : 0, iso-C15 : 0, C16 : 0 and iso-C16 : 0, which supported the allocation of the strain to the genus Gracilibacillus. The digital DNA-DNA hybridization value between strain TD8T and Gracilibacillus ureilyticus CGMCC 1.7727T was lower than 70 % (22.60 %), and the average nucleotide identity score was 79.54±5.09 %, suggesting that strain TD8T represented a novel species in the genus Gracilibacillus. The genomic DNA G+C content was 37.5 %. Based on physiological and biochemical characteristics and genotypic data, strain TD8T represents a novel species of the genus Gracilibacillus, for which the name Gracilibacillus oryzae sp. nov. is proposed. The type strain is TD8T (=ACCC 61556T=CICC 24889T=JCM 33537T). |
| 巻・号 | 70(10) |
| ページ | 5467-5472 |
| 公開日 | 2020-10-1 |
| DOI | 10.1099/ijsem.0.004427 |
| PMID | 32894210 |
| MeSH | Bacillaceae / classification* Bacillaceae / isolation & purification Bacterial Typing Techniques Base Composition China DNA, Bacterial / genetics Fatty Acids / chemistry Nucleic Acid Hybridization Oryza / microbiology* Phospholipids / chemistry Phylogeny* RNA, Ribosomal, 16S / genetics Seeds / microbiology* Sequence Analysis, DNA Vitamin K 2 / analogs & derivatives Vitamin K 2 / chemistry |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Blogs |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM33537 |