論文 - 詳細
| RRC ID | 66860 |
|---|---|
| 著者 | Takeda Y, Yamazaki K, Hashimoto K, Watanabe K, Chinen T, Kitagawa D. |
| タイトル | The centriole protein CEP76 negatively regulates PLK1 activity in the cytoplasm for proper mitotic progression. |
| ジャーナル | J Cell Sci |
| Abstract |
Polo-like kinase 1 (PLK1) dynamically changes its localization and plays important roles in proper mitotic progression. In particular, strict control of cytoplasmic PLK1 is needed to prevent mitotic defects. However, the regulation of cytoplasmic PLK1 is not fully understood. In this study, we show that CEP76, a centriolar protein, physically interacts with PLK1 and tightly controls the activation of cytoplasmic PLK1 during mitosis in human cells. We found that removal of centrosomes induced ectopic aggregation of PLK1, which is highly phosphorylated, in the cytoplasm during mitosis. Importantly, a targeted RNAi screen revealed that depletion of CEP76 resulted in a similar phenotype. In addition, depletion of CEP76 caused defective spindle orientation and mitotic delay. Moreover, the formation of ectopic PLK1 aggregates and defective spindle orientation were significantly suppressed by the inhibition of PLK1 kinase activity. Overall, these results demonstrate that CEP76 suppresses the aberrant activation of cytoplasmic PLK1 for proper mitotic progression.This article has an associated First Person interview with the first author of the paper. |
| 巻・号 | 133(19) |
| 公開日 | 2020-10-1 |
| DOI | 10.1242/jcs.241281 |
| PII | jcs.241281 |
| PMID | 32878946 |
| MeSH | Cell Cycle Proteins / genetics* Centrioles* / genetics Centrioles* / metabolism Centrosome / metabolism HeLa Cells Humans Microtubule-Associated Proteins / genetics* Mitosis / genetics Phosphorylation Protein Serine-Threonine Kinases / genetics Protein Serine-Threonine Kinases / metabolism Proto-Oncogene Proteins / genetics Spindle Apparatus* / genetics Spindle Apparatus* / metabolism |
| IF | 4.573 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | PANC-1(RCB2095) |