| RRC ID |
67270
|
| 著者 |
Zhang XF, Chao J, Pan QZ, Pan K, Weng DS, Wang QJ, Zhao JJ, He J, Liu Q, Jiang SS, Chen CL, Zhang HX, Xia JC.
|
| タイトル |
Overexpression of WWP1 promotes tumorigenesis and predicts unfavorable prognosis in patients with hepatocellular carcinoma.
|
| ジャーナル |
Oncotarget
|
| Abstract |
WW domain-containing E3 ubiquitin protein ligase 1 (WWP1) has been speculated to play important roles in the development of several kinds of cancers. However, the role of WWP1 in hepatocellular carcinoma(HCC) is not clear. In the present study, we investigated the expression and prognostic role of WWP1 in primary hepatocellular carcinoma (HCC) using cell lines and 149 archived HCC samples. Correlation between the functions of WWP1 in HCC was also explored. We used human HCC cell lines (BEL-7402, SMMC-7721, Hep-G2, Hep-3B, SK-hep1 and Huh7) and a normal hepatocyte cell line (LO2) along with HCC samples from patients who had undergone resection for HCC previously at our hospital. A battery of methods (real-time quantitative polymerase chain reaction; western blotting; immunohistochemical analyses; cell proliferation and colony formation assays; cell migration and cell invasion assays) were employed to assess various aspects of WWP1. We found that WWP1 expression was upregulated aberrantly at mRNA and protein levels in human primary HCC tissues. Amplified expression of WWP1 was highly correlated with poor outcome. Silencing of WWP1 expression by siRNA inhibited the proliferation, colony formation, migration and invasion of HCC cells in vitro, and resulted in significant apoptosis and cycle arrest in HCC cells. Our findings suggest that WWP1 might have an oncogenic role in human primary HCC, and that it could be used as a prognostic marker as well as a potential molecular target for the treatment of HCC.
|
| 巻・号 |
6(38)
|
| ページ |
40920-33
|
| 公開日 |
2015-12-1
|
| DOI |
10.18632/oncotarget.5712
|
| PII |
5712
|
| PMID |
26506518
|
| PMC |
PMC4747378
|
| MeSH |
Adolescent
Adult
Aged
Apoptosis
Biomarkers, Tumor / genetics
Biomarkers, Tumor / metabolism*
Blotting, Western
Carcinoma, Hepatocellular / genetics
Carcinoma, Hepatocellular / metabolism
Carcinoma, Hepatocellular / pathology*
Cell Movement
Cell Proliferation
Cell Transformation, Neoplastic / metabolism
Cell Transformation, Neoplastic / pathology*
Female
Follow-Up Studies
Gene Expression Regulation, Neoplastic*
Humans
Immunoenzyme Techniques
Liver Neoplasms / genetics
Liver Neoplasms / metabolism
Liver Neoplasms / pathology*
Male
Middle Aged
Neoplasm Grading
Neoplasm Invasiveness
Neoplasm Staging
Prognosis
RNA, Messenger / genetics
RNA, Small Interfering / genetics
Real-Time Polymerase Chain Reaction
Reverse Transcriptase Polymerase Chain Reaction
Survival Rate
Tumor Cells, Cultured
Ubiquitin-Protein Ligases / antagonists & inhibitors
Ubiquitin-Protein Ligases / genetics
Ubiquitin-Protein Ligases / metabolism*
Young Adult
|
| IF |
5.168
|
| リソース情報 |
| ヒト・動物細胞 |
HuH-7(RCB1366) |
