| RRC ID |
6728
|
| 著者 |
MacWilliams H, Doquang K, Pedrola R, Dollman G, Grassi D, Peis T, Tsang A, Ceccarelli A.
|
| タイトル |
A retinoblastoma ortholog controls stalk/spore preference in Dictyostelium.
|
| ジャーナル |
Development
|
| Abstract |
We describe rblA, the Dictyostelium ortholog of the retinoblastoma susceptibility gene Rb. In the growth phase, rblA expression is correlated with several factors that lead to 'preference' for the spore pathway. During multicellular development, expression increases 200-fold in differentiating spores. rblA-null strains differentiate stalk cells and spores normally, but in chimeras with wild type, the mutant shows a strong preference for the stalk pathway. rblA-null cells are hypersensitive to the stalk morphogen DIF, suggesting that rblA normally suppresses the DIF response in cells destined for the spore pathway. rblA overexpression during growth leads to G1 arrest, but as growing Dictyostelium are overwhelmingly in G2 phase, rblA does not seem to be important in the normal cell cycle. rblA-null cells show reduced cell size and a premature growth-development transition; the latter appears anomalous but may reflect selection pressures acting on social ameba.
|
| 巻・号 |
133(7)
|
| ページ |
1287-97
|
| 公開日 |
2006-4-1
|
| DOI |
10.1242/dev.02287
|
| PII |
dev.02287
|
| PMID |
16495312
|
| MeSH |
Amino Acid Sequence
Animals
Cell Cycle
Cell Differentiation
Cell Nucleus / chemistry
Cell Size
Conserved Sequence
DNA, Protozoan / analysis
Dictyostelium / cytology*
Dictyostelium / genetics
Dictyostelium / growth & development*
Dose-Response Relationship, Drug
Evolution, Molecular
Fluorescent Dyes
Gene Expression Regulation / drug effects
Genes, Protozoan*
Genes, Retinoblastoma*
Glucose / pharmacology
Hexanones / pharmacology
Indoles
Molecular Sequence Data
Mutation
Promoter Regions, Genetic
Protein Structure, Tertiary
Protozoan Proteins / genetics
Protozoan Proteins / physiology*
Sequence Homology, Amino Acid
Spores, Protozoan*
|
| IF |
5.611
|
| 引用数 |
25
|
|
WOS 分野
|
DEVELOPMENTAL BIOLOGY
|
| リソース情報 |
| 細胞性粘菌 |
|
