論文 - 詳細
| RRC ID | 67494 |
|---|---|
| 著者 | Inamura SI, Tanabe T, Kawamukai M, Matsuo Y. |
| タイトル | Expression of Mug14 is regulated by the transcription factor Rst2 through the cAMP-dependent protein kinase pathway in Schizosaccharomyces pombe. |
| ジャーナル | Curr Genet |
| Abstract |
The cAMP-dependent protein kinase (Pka1) regulates many cellular events, including sexual development and glycogenesis, and response to the limitation of glucose, in Schizosaccharomyces pombe. Despite its importance in many cellular events, the targets of the cAMP/PKA pathway have not been fully investigated. Here, we demonstrate that the expression of mug14 is induced by downregulation of the cAMP/PKA pathway and limitation of glucose. This regulation is dependent on the function of Rst2, a transcription factor that regulates transition from mitosis to meiosis. The loss of the C2H2-type zinc finger domain in Rst2, termed Rst2 (C2H2∆), abolished the induction of Mug14 expression. Upon deletion of the stress starvation response element of the S. pombe (STREP: CCCCTC) sequence, which is a potential binding site of Rst2 on mug14, in the pka1∆ strain, its induction was abolished. The expression of Mug14 was significantly reduced and delayed by the limitation of glucose and also by nitrogen starvation in the rst2∆ strain. Mug14 is known to share a common function with Mde1 and Mta3 in the methionine salvage pathway, but the expression of mde1 and mta3 mRNAs was not enhanced by pka1 deletion and limitation of glucose. We conclude that the expression of Mug14 is upregulated by Rst2 under the control of the cAMP/PKA signaling pathway, which senses the limitation of glucose. |
| 巻・号 | 67(5) |
| ページ | 807-821 |
| 公開日 | 2021-10-1 |
| DOI | 10.1007/s00294-021-01194-z |
| PII | 10.1007/s00294-021-01194-z |
| PMID | 34086083 |
| MeSH | Cell Cycle Proteins / genetics* Cyclic AMP-Dependent Protein Kinases / physiology Gene Expression Regulation, Fungal* Glucose / metabolism Green Fluorescent Proteins / genetics MAP Kinase Signaling System Nitrogen / metabolism Nucleic Acid Conformation Promoter Regions, Genetic RNA, Fungal RNA, Messenger Recombinant Fusion Proteins / genetics Schizosaccharomyces / enzymology Schizosaccharomyces / genetics* Schizosaccharomyces pombe Proteins / genetics* Schizosaccharomyces pombe Proteins / physiology Stress, Physiological Transcription Factors / physiology* |
| IF | 3.464 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 酵母 | FY21846 (mug14) |