RRC ID 67728
Author Kishimoto M, Uemura K, Sanaki T, Sato A, Hall WW, Kariwa H, Orba Y, Sawa H, Sasaki M.
Title TMPRSS11D and TMPRSS13 Activate the SARS-CoV-2 Spike Protein.
Journal Viruses
Abstract Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) utilizes host proteases, including a plasma membrane-associated transmembrane protease, serine 2 (TMPRSS2) to cleave and activate the virus spike protein to facilitate cellular entry. Although TMPRSS2 is a well-characterized type II transmembrane serine protease (TTSP), the role of other TTSPs on the replication of SARS-CoV-2 remains to be elucidated. Here, we have screened 12 TTSPs using human angiotensin-converting enzyme 2-expressing HEK293T (293T-ACE2) cells and Vero E6 cells and demonstrated that exogenous expression of TMPRSS11D and TMPRSS13 enhanced cellular uptake and subsequent replication of SARS-CoV-2. In addition, SARS-CoV-1 and SARS-CoV-2 share the same TTSPs in the viral entry process. Our study demonstrates the impact of host TTSPs on infection of SARS-CoV-2, which may have implications for cell and tissue tropism, for pathogenicity, and potentially for vaccine development.
Volume 13(3)
Published 2021-2-28
DOI 10.3390/v13030384
PII v13030384
PMID 33671076
PMC PMC8001073
MeSH Angiotensin-Converting Enzyme 2 / metabolism Animals COVID-19 / metabolism* COVID-19 / virology* Chlorocebus aethiops HEK293 Cells Humans Membrane Proteins / metabolism* SARS-CoV-2 / metabolism* Serine Endopeptidases / metabolism* Serine Proteases / metabolism* Spike Glycoprotein, Coronavirus / metabolism* Vero Cells Virus Internalization
IF 3.816