RRC ID 68064
Author Yamagishi M, Kubokawa M, Kuze Y, Suzuki A, Yokomizo A, Kobayashi S, Nakashima M, Makiyama J, Iwanaga M, Fukuda T, Watanabe T, Suzuki Y, Uchimaru K.
Title Chronological genome and single-cell transcriptome integration characterizes the evolutionary process of adult T cell leukemia-lymphoma.
Journal Nat Commun
Abstract Subclonal genetic heterogeneity and their diverse gene expression impose serious problems in understanding the behavior of cancers and contemplating therapeutic strategies. Here we develop and utilize a capture-based sequencing panel, which covers host hotspot genes and the full-length genome of human T-cell leukemia virus type-1 (HTLV-1), to investigate the clonal architecture of adult T-cell leukemia-lymphoma (ATL). For chronologically collected specimens from patients with ATL or pre-onset individuals, we integrate deep DNA sequencing and single-cell RNA sequencing to detect the somatic mutations and virus directly and characterize the transcriptional readouts in respective subclones. Characteristic genomic and transcriptomic patterns are associated with subclonal expansion and switches during the clinical timeline. Multistep mutations in the T-cell receptor (TCR), STAT3, and NOTCH pathways establish clone-specific transcriptomic abnormalities and further accelerate their proliferative potential to develop highly malignant clones, leading to disease onset and progression. Early detection and characterization of newly expanded subclones through the integrative analytical platform will be valuable for the development of an in-depth understanding of this disease.
Volume 12(1)
Pages 4821
Published 2021-8-10
DOI 10.1038/s41467-021-25101-9
PII 10.1038/s41467-021-25101-9
PMID 34376672
PMC PMC8355240
MeSH Adult Cell Line, Tumor Cell Proliferation / genetics Cells, Cultured Clonal Evolution / genetics Clone Cells / metabolism Clone Cells / pathology Genome, Viral / genetics* HTLV-I Infections / virology Human T-lymphotropic virus 1 / genetics* Human T-lymphotropic virus 1 / physiology Humans Jurkat Cells Leukemia-Lymphoma, Adult T-Cell / genetics* Leukemia-Lymphoma, Adult T-Cell / pathology Leukemia-Lymphoma, Adult T-Cell / virology Mutation RNA-Seq / methods Receptor, Notch1 / genetics Receptors, Antigen, T-Cell / genetics STAT3 Transcription Factor / genetics Single-Cell Analysis / methods* Transcriptome / genetics*
IF 12.121
DNA material CSII-EF-MCS-IRES2-Venus (RDB04384) pCMV-VSV-G-RSV-Rev (RDB04393) pCAG-HIVgp (RDB04394)
Human and Animal Cells Jurkat(RCB3052)