論文 - 詳細
| RRC ID | 68072 |
|---|---|
| 著者 | Fajner V, Giavazzi F, Sala S, Oldani A, Martini E, Napoletano F, Parazzoli D, Cesare G, Cerbino R, Maspero E, Vaccari T, Polo S. |
| タイトル | Hecw controls oogenesis and neuronal homeostasis by promoting the liquid state of ribonucleoprotein particles. |
| ジャーナル | Nat Commun |
| Abstract |
Specialised ribonucleoprotein (RNP) granules are a hallmark of polarized cells, like neurons and germ cells. Among their main functions is the spatial and temporal modulation of the activity of specific mRNA transcripts that allow specification of primary embryonic axes. While RNPs composition and role are well established, their regulation is poorly defined. Here, we demonstrate that Hecw, a newly identified Drosophila ubiquitin ligase, is a key modulator of RNPs in oogenesis and neurons. Hecw depletion leads to the formation of enlarged granules that transition from a liquid to a gel-like state. Loss of Hecw activity results in defective oogenesis, premature aging and climbing defects associated with neuronal loss. At the molecular level, reduced ubiquitination of the Fmrp impairs its translational repressor activity, resulting in altered Orb expression in nurse cells and Profilin in neurons. |
| 巻・号 | 12(1) |
| ページ | 5488 |
| 公開日 | 2021-9-16 |
| DOI | 10.1038/s41467-021-25809-8 |
| PII | 10.1038/s41467-021-25809-8 |
| PMID | 34531401 |
| PMC | PMC8446043 |
| MeSH | Animals Cytoplasmic Granules / metabolism Drosophila Proteins / genetics* Drosophila Proteins / metabolism Drosophila melanogaster / cytology Drosophila melanogaster / genetics* Drosophila melanogaster / growth & development Drosophila melanogaster / metabolism Embryo, Nonmammalian Fragile X Mental Retardation Protein / genetics Fragile X Mental Retardation Protein / metabolism Gene Expression Regulation, Developmental* Homeostasis / genetics Longevity / genetics Neurogenesis / genetics* Neurons / cytology Neurons / metabolism Oocytes / cytology Oocytes / metabolism Oogenesis / genetics* Phase Transition Profilins / genetics Profilins / metabolism Protein Biosynthesis Protein Processing, Post-Translational RNA-Binding Proteins / genetics RNA-Binding Proteins / metabolism Ribonucleoproteins / genetics* Ribonucleoproteins / metabolism Ubiquitin-Protein Ligases / genetics* Ubiquitin-Protein Ligases / metabolism Ubiquitination |
| IF | 12.121 |
| リソース情報 | |
| ショウジョウバエ | pBFvU6.2 DGRC#104055 |