論文 - 詳細
| RRC ID | 68428 |
|---|---|
| 著者 | Abe Y, Nakao H, Goto M, Tamano M, Koebis M, Nakao K, Aiba A. |
| タイトル | Efficient marmoset genome engineering by autologous embryo transfer and CRISPR/Cas9 technology. |
| ジャーナル | Sci Rep |
| Abstract |
Genetic engineering of non-human primates, which are most closely related to humans, has been expected to generate ideal animal models for human genetic diseases. The common marmoset (Callithrix jacchus) is a non-human primate species adequate for the production of genetically modified animals because of their small body size and high reproductive capacity. Autologous embryo transfer (AET) is routinely utilized in assisted reproductive technologies for humans but not for experimental animals. This study has developed a novel method for efficiently producing mutant marmosets using AET and CRISPR/Cas9 systems. The embryos were recovered from oviducts of naturally mated females, injected with Cas9/guide RNA, and transferred into the oviducts of the donors. This AET method can reduce the time for in vitro culture of embryos to less than 30 min. This method uses an embryo donor as the recipient, thus reducing the number of animals and allowing for "Reduction" in the 3R principles of humane experimental technique. Furthermore, this method can utilize nulliparous females as well as parous females. We applied our novel method and generated the 6 marmosets carrying mutations in the fragile X mental retardation 1 (FMR1) gene using only 18 females including 14 nulliparous females. |
| 巻・号 | 11(1) |
| ページ | 20234 |
| 公開日 | 2021-10-12 |
| DOI | 10.1038/s41598-021-99656-4 |
| PII | 10.1038/s41598-021-99656-4 |
| PMID | 34642413 |
| PMC | PMC8511084 |
| MeSH | Animals Autografts CRISPR-Cas Systems Callithrix / genetics* Embryo Culture Techniques Embryo Transfer / methods* Female Fragile X Mental Retardation Protein / genetics* Genetic Engineering / methods* Models, Animal Mutation |
| IF | 3.998 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | CMES40(AES0166) |