RRC ID 693
Author Kishii M, Yamada T, Sasakuma T, Tsujimoto H.
Title Production of wheat-Leymus racemosus chromosome addition lines.
Journal Theor Appl Genet
Abstract We produced ten wheat-Leymus racemosus chromosome addition lines. Eight chromosomes (A, C, F, H, I, J, k, and l) were recovered as disomic additions and two (E and n) as monosomic. Screening of the addition lines was done by fluorescence in situ hybridization using several repetitive sequences as probes, which allowed us to identify different L. racemosus chromosomes and find many aberrant L. racemosus chromosomes. RFLP analysis revealed partial conservation of homology between L. racemosus and wheat chromosomes, depending on the homologous groups. Chromosomes A and l belonged to group 2, chromosomes C and I to group 5, and chromosome k to group 6. Chromosomes H and J were a mixture of groups 1, 3, and 7, chromosome n of groups 3 and 7, and chromosomes E and F were of group 4 and others. Comparison of our addition lines with other addition lines showed large cytological differences.
Volume 109(2)
Pages 255-60
Published 2004-7-1
DOI 10.1007/s00122-004-1631-y
PMID 15057417
MeSH Blotting, Southern Chromosomes, Plant / genetics* In Situ Hybridization, Fluorescence Plants, Genetically Modified Poaceae / genetics* Polymorphism, Restriction Fragment Length Synteny / genetics Triticum / genetics*
IF 4.439
Times Cited 34
WOS Category AGRONOMY HORTICULTURE PLANT SCIENCES GENETICS & HEREDITY
Resource
Wheat Le. racemosus chromosome addition lines KT020-003