論文 - 詳細
| RRC ID | 6945 |
|---|---|
| 著者 | Takos A, Lai D, Mikkelsen L, Abou Hachem M, Shelton D, Motawia MS, Olsen CE, Wang TL, Martin C, Rook F. |
| タイトル | Genetic screening identifies cyanogenesis-deficient mutants of Lotus japonicus and reveals enzymatic specificity in hydroxynitrile glucoside metabolism. |
| ジャーナル | Plant Cell |
| Abstract |
Cyanogenesis, the release of hydrogen cyanide from damaged plant tissues, involves the enzymatic degradation of amino acid-derived cyanogenic glucosides (alpha-hydroxynitrile glucosides) by specific beta-glucosidases. Release of cyanide functions as a defense mechanism against generalist herbivores. We developed a high-throughput screening method and used it to identify cyanogenesis deficient (cyd) mutants in the model legume Lotus japonicus. Mutants in both biosynthesis and catabolism of cyanogenic glucosides were isolated and classified following metabolic profiling of cyanogenic glucoside content. L. japonicus produces two cyanogenic glucosides: linamarin (derived from Val) and lotaustralin (derived from Ile). Their biosynthesis may involve the same set of enzymes for both amino acid precursors. However, in one class of mutants, accumulation of lotaustralin and linamarin was uncoupled. Catabolic mutants could be placed in two complementation groups, one of which, cyd2, encoded the beta-glucosidase BGD2. Despite the identification of nine independent cyd2 alleles, no mutants involving the gene encoding a closely related beta-glucosidase, BGD4, were identified. This indicated that BGD4 plays no role in cyanogenesis in L. japonicus in vivo. Biochemical analysis confirmed that BGD4 cannot hydrolyze linamarin or lotaustralin and in L. japonicus is specific for breakdown of related hydroxynitrile glucosides, such as rhodiocyanoside A. By contrast, BGD2 can hydrolyze both cyanogenic glucosides and rhodiocyanosides. Our genetic analysis demonstrated specificity in the catabolic pathways for hydroxynitrile glucosides and implied specificity in their biosynthetic pathways as well. In addition, it has provided important tools for elucidating and potentially modifying cyanogenesis pathways in plants. |
| 巻・号 | 22(5) |
| ページ | 1605-19 |
| 公開日 | 2010-5-1 |
| DOI | 10.1105/tpc.109.073502 |
| PII | tpc.109.073502 |
| PMID | 20453117 |
| PMC | PMC2899875 |
| MeSH | Alleles Biological Assay Genes, Plant / genetics Genetic Complementation Test Genetic Testing* Glycosides / biosynthesis Glycosides / chemistry Glycosides / metabolism* High-Throughput Screening Assays Hydrogen Cyanide / metabolism* Hydrolysis Lotus / enzymology* Lotus / genetics* Metabolome Models, Molecular Molecular Sequence Data Mutation / genetics* Phenotype Plant Leaves / enzymology Plant Proteins / chemistry Plant Proteins / genetics Plant Proteins / metabolism* Plants, Genetically Modified Substrate Specificity Tobacco / metabolism |
| IF | 9.618 |
| 引用数 | 43 |
| WOS 分野 | PLANT SCIENCES BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ミヤコグサ・ダイズ | Miyakojima MG-20 |