| RRC ID |
69465
|
| 著者 |
Saito K, Mitsui A, Sumardika IW, Yokoyama Y, Sakaguchi M, Kondo E.
|
| タイトル |
PLOD2-driven IL-6/STAT3 signaling promotes the invasion and metastasis of oral squamous cell carcinoma via activation of integrin β1.
|
| ジャーナル |
Int J Oncol
|
| Abstract |
We previously reported that high expression of procollagen‑lysine 2‑oxoglutarate 5‑dioxygenase 2 (PLOD2) leads to stabilization and plasma membrane translocation of integrin β1 to promote the invasion and metastasis of oral squamous cell carcinoma (SCC). The present study aimed to further understand the relationship between PLOD2‑integrin β1 signaling and the tumor microenvironment. This study provided further advanced insights indicating that elevated interleukin (IL)‑6 in the tumor microenvironment acts as a key molecule that triggers PLOD2‑integrin β1 axis‑derived acceleration of tumor invasion and metastasis. It was found using the dual‑luciferase reporter assay system that signal transducer and activator of transcription 3 (STAT3) activation by IL‑6 was essential for increasing the expression levels of PLOD2 through direct activation of the PLOD2 promoter in oral SCC, whereas IL‑6 stimulation did not contribute to integrin β1 expression or the subsequent maturation process towards a functional form on the plasma membrane. Furthermore, the expression of IL‑6 in oral SCC tissues was mainly observed in the tumor stroma. Finally, with double immunofluorescence staining, it was found that IL‑6 expression occurred in CD163‑positive M2 macrophages distributed around the tumor nest. These results combined with our previous results indicate that as IL‑6 significantly increases STAT3‑mediated PLOD2 promoter activity, IL‑6 released by M2‑type tumor‑associated macrophages is a crucial factor that promotes PLOD2‑integrin β1 axis‑enhanced invasion and metastasis of oral SCC cells.
|
| 巻・号 |
58(6)
|
| 公開日 |
2021-6-1
|
| DOI |
10.3892/ijo.2021.5209
|
| PII |
29
|
| PMID |
33887877
|
| PMC |
PMC8057293
|
| MeSH |
Cell Line, Tumor
Cell Movement / genetics
Gene Expression Regulation, Neoplastic
Humans
Integrin beta1 / metabolism*
Interleukin-6 / metabolism*
Mouth Neoplasms / genetics*
Mouth Neoplasms / immunology
Mouth Neoplasms / pathology
Neoplasm Invasiveness / genetics
Neoplasm Invasiveness / immunology
Neoplasm Invasiveness / pathology
Procollagen-Lysine, 2-Oxoglutarate 5-Dioxygenase / genetics*
Procollagen-Lysine, 2-Oxoglutarate 5-Dioxygenase / metabolism
Promoter Regions, Genetic / genetics
STAT3 Transcription Factor / metabolism
Signal Transduction / genetics
Signal Transduction / immunology
Squamous Cell Carcinoma of Head and Neck / genetics*
Squamous Cell Carcinoma of Head and Neck / immunology
Squamous Cell Carcinoma of Head and Neck / pathology
Tumor Microenvironment / genetics
Tumor Microenvironment / immunology
Tumor-Associated Macrophages / immunology
Tumor-Associated Macrophages / metabolism
|
| IF |
3.899
|
| リソース情報 |
| ヒト・動物細胞 |
HSC-2(RCB1945)
HSC-3(RCB1975)
Ca9-22(RCB1976) |
