RRC ID 6983
Author Suzaki T, Ohneda M, Toriba T, Yoshida A, Hirano HY.
Title FON2 SPARE1 redundantly regulates floral meristem maintenance with FLORAL ORGAN NUMBER2 in rice.
Journal PLoS Genet.
Abstract CLAVATA signaling restricts stem cell identity in the shoot apical meristem (SAM) in Arabidopsis thaliana. In rice (Oryza sativa), FLORAL ORGAN NUMBER2 (FON2), closely related to CLV3, is involved as a signaling molecule in a similar pathway to negatively regulate stem cell proliferation in the floral meristem (FM). Here we show that the FON2 SPARE1 (FOS1) gene encoding a CLE protein functions along with FON2 in maintenance of the FM. In addition, FOS1 appears to be involved in maintenance of the SAM in the vegetative phase, because constitutive expression of FOS1 caused termination of the vegetative SAM. Genetic analysis revealed that FOS1 does not need FON1, the putative receptor of FON2, for its action, suggesting that FOS1 and FON2 may function in meristem maintenance as signaling molecules in independent pathways. Initially, we identified FOS1 as a suppressor that originates from O. sativa indica and suppresses the fon2 mutation in O. sativa japonica. FOS1 function in japonica appears to be compromised by a functional nucleotide polymorphism (FNP) at the putative processing site of the signal peptide. Sequence comparison of FOS1 in about 150 domesticated rice and wild rice species indicates that this FNP is present only in japonica, suggesting that redundant regulation by FOS1 and FON2 is commonplace in species in the Oryza genus. Distribution of the FNP also suggests that this mutation may have occurred during the divergence of japonica from its wild ancestor. Stem cell maintenance may be regulated by at least three negative pathways in rice, and each pathway may contribute differently to this regulation depending on the type of the meristem. This situation contrasts with that in Arabidopsis, where CLV signaling is the major single pathway in all meristems.
Volume 5(10)
Pages e1000693
Published 2009-10
DOI 10.1371/journal.pgen.1000693
PMID 19834537
PMC PMC2752996
MeSH Amino Acid Sequence Conserved Sequence Flowers / genetics* Gene Expression Regulation, Plant* Genome, Plant Meristem / genetics* Molecular Sequence Data Mutation Oryza / chemistry Oryza / genetics* Plant Proteins / chemistry Plant Proteins / genetics* Polymorphism, Genetic Sequence Alignment
IF 5.54
Times Cited 22
WOS Category GENETICS & HEREDITY
Resource
Rice W0106 W1866 W2263 W1236 W1807 W1945 W2078 W1294 W2003 W0120 W2051 W0630 W1921 W1169=W1165 W2145 W2199 W1413 W1508 C7599 C8538(B) W1588 W0652 W1625 W1635