論文 - 詳細
| RRC ID | 69831 |
|---|---|
| 著者 | Haniu H, Tsukahara T, Matsuda Y, Usui Y, Aoki K, Shimizu M, Ogihara N, Hara K, Takanashi S, Okamoto M, Ishigaki N, Nakamura K, Kato H, Saito N. |
| タイトル | DJ-1 as a potential biomarker for the development of biocompatible multiwalled carbon nanotubes. |
| ジャーナル | Int J Nanomedicine |
| Abstract |
BACKGROUND:In the present study, we investigated whether DJ-1 could serve as a biomarker for assessing the biocompatibility of multiwalled carbon nanotubes (MWCNTs), using the highly purified carbon nanotube, HTT2800. METHODS:Using Western blot analysis, we determined DJ-1 protein levels in two different types of cells (one capable and the other incapable of HTT2800 endocytosis). Using quantitative real-time polymerase chain reaction, we also investigated the ability of purified nanotubes to alter DJ-1 mRNA levels. RESULTS:We demonstrated that the DJ-1 protein concentration was reduced, regardless of the cytotoxic activity of intracellular HTT2800. Furthermore, HTT2800 decreased the DJ-1 mRNA levels in a dose-dependent manner. This decrease in DJ-1 mRNA levels was not observed in the case of Sumi black or cup-stacked carbon nanotubes. CONCLUSION:These data indicate that modification of DJ-1 expression is caused by the cell response to MWCNTs. We conclude that DJ-1 is a promising candidate biomarker for the development of biocompatible MWCNTs. |
| 巻・号 | 6 |
| ページ | 2689-95 |
| 公開日 | 2011-1-1 |
| DOI | 10.2147/IJN.S25471 |
| PII | ijn-6-2689 |
| PMID | 22114499 |
| PMC | PMC3218582 |
| MeSH | Biocompatible Materials / chemistry* Biocompatible Materials / pharmacology Biomarkers / analysis Cell Line, Tumor Cell Survival Down-Regulation / drug effects Humans Intracellular Signaling Peptides and Proteins / analysis* Intracellular Signaling Peptides and Proteins / genetics Intracellular Signaling Peptides and Proteins / metabolism Materials Testing / methods* Nanotubes, Carbon / chemistry* Oncogene Proteins / analysis* Oncogene Proteins / genetics Oncogene Proteins / metabolism Protein Deglycase DJ-1 RNA, Messenger / analysis Real-Time Polymerase Chain Reaction |
| IF | 5.115 |
| リソース情報 | |
| ヒト・動物細胞 | THP-1(RCB1189) ACC-MESO-1(RCB2292) |