RRC ID 6987
著者 Shi A, Chen CC, Banerjee R, Glodowski D, Audhya A, Rongo C, Grant BD.
タイトル EHBP-1 functions with RAB-10 during endocytic recycling in Caenorhabditis elegans.
ジャーナル Mol Biol Cell
Abstract Caenorhabditis elegans RAB-10 functions in endocytic recycling in polarized cells, regulating basolateral cargo transport in the intestinal epithelia and postsynaptic cargo transport in interneurons. A similar role was found for mammalian Rab10 in MDCK cells, suggesting that a conserved mechanism regulates these related pathways in metazoans. In a yeast two-hybrid screen for binding partners of RAB-10 we identified EHBP-1, a calponin homology domain (CH) protein, whose mammalian homolog Ehbp1 was previously shown to function during endocytic transport of GLUT4 in adipocytes. In vivo we find that EHBP-1-GFP colocalizes with RFP-RAB-10 on endosomal structures of the intestine and interneurons and that ehbp-1 loss-of-function mutants share with rab-10 mutants specific endosome morphology and cargo localization defects. We also show that loss of EHBP-1 disrupts transport of membrane proteins to the plasma membrane of the nonpolarized germline cells, a defect that can be phenocopied by codepletion of RAB-10 and its closest paralog RAB-8. These results indicate that RAB-10 and EHBP-1 function together in many cell types and suggests that there are differences in the level of redundancy among Rab family members in polarized versus nonpolarized cells.
巻・号 21(16)
ページ 2930-43
公開日 2010-8-15
DOI 10.1091/mbc.E10-02-0149
PII E10-02-0149
PMID 20573983
PMC PMC2921114
MeSH Adenosine Triphosphate / metabolism Amino Acid Sequence Animals Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism Caenorhabditis elegans / physiology* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Endocytosis / genetics Endocytosis / physiology* Endosomes / metabolism Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Microscopy, Fluorescence Molecular Sequence Data Protein Binding Protein Transport RNA Interference Receptors, AMPA / genetics Receptors, AMPA / metabolism Sequence Homology, Amino Acid Two-Hybrid System Techniques Vesicular Transport Proteins / genetics Vesicular Transport Proteins / metabolism* rab GTP-Binding Proteins / genetics rab GTP-Binding Proteins / metabolism*
IF 3.791
引用数 56
WOS 分野 CELL BIOLOGY
リソース情報
線虫 tm2523