RRC ID |
6987
|
著者 |
Shi A, Chen CC, Banerjee R, Glodowski D, Audhya A, Rongo C, Grant BD.
|
タイトル |
EHBP-1 functions with RAB-10 during endocytic recycling in Caenorhabditis elegans.
|
ジャーナル |
Mol Biol Cell
|
Abstract |
Caenorhabditis elegans RAB-10 functions in endocytic recycling in polarized cells, regulating basolateral cargo transport in the intestinal epithelia and postsynaptic cargo transport in interneurons. A similar role was found for mammalian Rab10 in MDCK cells, suggesting that a conserved mechanism regulates these related pathways in metazoans. In a yeast two-hybrid screen for binding partners of RAB-10 we identified EHBP-1, a calponin homology domain (CH) protein, whose mammalian homolog Ehbp1 was previously shown to function during endocytic transport of GLUT4 in adipocytes. In vivo we find that EHBP-1-GFP colocalizes with RFP-RAB-10 on endosomal structures of the intestine and interneurons and that ehbp-1 loss-of-function mutants share with rab-10 mutants specific endosome morphology and cargo localization defects. We also show that loss of EHBP-1 disrupts transport of membrane proteins to the plasma membrane of the nonpolarized germline cells, a defect that can be phenocopied by codepletion of RAB-10 and its closest paralog RAB-8. These results indicate that RAB-10 and EHBP-1 function together in many cell types and suggests that there are differences in the level of redundancy among Rab family members in polarized versus nonpolarized cells.
|
巻・号 |
21(16)
|
ページ |
2930-43
|
公開日 |
2010-8-15
|
DOI |
10.1091/mbc.E10-02-0149
|
PII |
E10-02-0149
|
PMID |
20573983
|
PMC |
PMC2921114
|
MeSH |
Adenosine Triphosphate / metabolism
Amino Acid Sequence
Animals
Caenorhabditis elegans / genetics
Caenorhabditis elegans / metabolism
Caenorhabditis elegans / physiology*
Caenorhabditis elegans Proteins / genetics
Caenorhabditis elegans Proteins / metabolism*
Endocytosis / genetics
Endocytosis / physiology*
Endosomes / metabolism
Green Fluorescent Proteins / genetics
Green Fluorescent Proteins / metabolism
Microscopy, Fluorescence
Molecular Sequence Data
Protein Binding
Protein Transport
RNA Interference
Receptors, AMPA / genetics
Receptors, AMPA / metabolism
Sequence Homology, Amino Acid
Two-Hybrid System Techniques
Vesicular Transport Proteins / genetics
Vesicular Transport Proteins / metabolism*
rab GTP-Binding Proteins / genetics
rab GTP-Binding Proteins / metabolism*
|
IF |
3.791
|
引用数 |
56
|
WOS 分野
|
CELL BIOLOGY
|
リソース情報 |
線虫 |
tm2523 |