Reference - Detail
| RRC ID | 7003 |
|---|---|
| Author | Hagi T, Kobayashi M, Nomura M. |
| Title | Molecular-based analysis of changes in indigenous milk microflora during the grazing period. |
| Journal | Biosci Biotechnol Biochem |
| Abstract |
Variations in milk microflora caused by changes in the cow feeding environment (from inside to outside grazing) were analyzed using a molecular-based approach comprising denaturing gradient gel electrophoresis and real-time PCR. After 8 d of outside grazing, changes in milk microflora were observed. Denaturing gradient gel electrophoresis analysis showed that the predominant bacterial group in the milk belonged to the Lactobacillus species during the experimental period, whereas the genus Staphylococcus gained in numbers during the outside grazing period in addition to Lactobacillus. To investigate the quantitative dynamics of staphylococci, real-time PCR was performed using staphylococcal-specific primers. Real-time PCR analysis revealed that the population of staphylococci increased during the outside grazing period. Our combined denaturing gradient gel electrophoresis and real-time PCR approach enables precise monitoring of the dynamics of both total bacteria and specific bacterial species in milk. Our results provide information on grazing management and the manufacture of dairy products. |
| Volume | 74(3) |
| Pages | 484-7 |
| Published | 2010-1-1 |
| DOI | 10.1271/bbb.90470 |
| PII | JST.JSTAGE/bbb/90470 |
| PMID | 20208366 |
| MeSH | Animal Feed / microbiology* Animals Bacterial Typing Techniques Cattle / microbiology Cattle / physiology* DNA, Bacterial / genetics Electrophoresis, Polyacrylamide Gel / methods Female Lactobacillaceae / classification* Lactobacillaceae / genetics Lactobacillaceae / isolation & purification Milk / microbiology* Polymerase Chain Reaction Staphylococcaceae / classification* Staphylococcaceae / genetics Staphylococcaceae / isolation & purification |
| IF | 1.516 |
| Times Cited | 19 |
| WOS Category | FOOD SCIENCE & TECHNOLOGY CHEMISTRY, APPLIED BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Resource | |
| General Microbes | JCM 2418 |