RRC ID 70324
Author Zeng CW, Kamei Y, Shigenobu S, Sheu JC, Tsai HJ.
Title Injury-induced Cavl-expressing cells at lesion rostral side play major roles in spinal cord regeneration.
Journal Open Biol
Abstract The extent of cellular heterogeneity involved in neuronal regeneration after spinal cord injury (SCI) remains unclear. Therefore, we established stress-responsive transgenic zebrafish embryos with SCI. As a result, we found an SCI-induced cell population, termed SCI stress-responsive regenerating cells (SrRCs), essential for neuronal regeneration post-SCI. SrRCs were mostly composed of subtypes of radial glia (RGs-SrRCs) and neuron stem/progenitor cells (NSPCs-SrRCs) that are able to differentiate into neurons, and they formed a bridge across the lesion and connected with neighbouring undamaged motor neurons post-SCI. Compared to SrRCs at the caudal side of the SCI site (caudal-SrRCs), rostral-SrRCs participated more actively in neuronal regeneration. After RNA-seq analysis, we discovered that caveolin 1 (cav1) was significantly upregulated in rostral-SrRCs and that cav1 was responsible for the axonal regrowth and regenerative capability of rostral-SrRCs. Collectively, we define a specific SCI-induced cell population, SrRCs, involved in neuronal regeneration, demonstrate that rostral-SrRCs exhibit higher neuronal differentiation capability and prove that cav1 is predominantly expressed in rostral-SrRCs, playing a major role in neuronal regeneration after SCI.
Volume 11(2)
Pages 200304
Published 2021-2-1
DOI 10.1098/rsob.200304
PMID 33622104
PMC PMC8061693
MeSH Animals Caveolin 1 / genetics Caveolin 1 / metabolism* Cells, Cultured Motor Neurons / cytology Motor Neurons / metabolism Neural Stem Cells / cytology Neural Stem Cells / metabolism Neuronal Outgrowth Spinal Cord Injuries / metabolism Spinal Cord Injuries / physiopathology Spinal Cord Regeneration* Up-Regulation Zebrafish Zebrafish Proteins / genetics Zebrafish Proteins / metabolism*
IF 4.931
Zebrafish Tg(gfap:dTomato)