論文 - 詳細
| RRC ID | 70324 |
|---|---|
| 著者 | Zeng CW, Kamei Y, Shigenobu S, Sheu JC, Tsai HJ. |
| タイトル | Injury-induced Cavl-expressing cells at lesion rostral side play major roles in spinal cord regeneration. |
| ジャーナル | Open Biol |
| Abstract |
The extent of cellular heterogeneity involved in neuronal regeneration after spinal cord injury (SCI) remains unclear. Therefore, we established stress-responsive transgenic zebrafish embryos with SCI. As a result, we found an SCI-induced cell population, termed SCI stress-responsive regenerating cells (SrRCs), essential for neuronal regeneration post-SCI. SrRCs were mostly composed of subtypes of radial glia (RGs-SrRCs) and neuron stem/progenitor cells (NSPCs-SrRCs) that are able to differentiate into neurons, and they formed a bridge across the lesion and connected with neighbouring undamaged motor neurons post-SCI. Compared to SrRCs at the caudal side of the SCI site (caudal-SrRCs), rostral-SrRCs participated more actively in neuronal regeneration. After RNA-seq analysis, we discovered that caveolin 1 (cav1) was significantly upregulated in rostral-SrRCs and that cav1 was responsible for the axonal regrowth and regenerative capability of rostral-SrRCs. Collectively, we define a specific SCI-induced cell population, SrRCs, involved in neuronal regeneration, demonstrate that rostral-SrRCs exhibit higher neuronal differentiation capability and prove that cav1 is predominantly expressed in rostral-SrRCs, playing a major role in neuronal regeneration after SCI. |
| 巻・号 | 11(2) |
| ページ | 200304 |
| 公開日 | 2021-2-1 |
| DOI | 10.1098/rsob.200304 |
| PMID | 33622104 |
| PMC | PMC8061693 |
| MeSH | Animals Caveolin 1 / genetics Caveolin 1 / metabolism* Cells, Cultured Motor Neurons / cytology Motor Neurons / metabolism Neural Stem Cells / cytology Neural Stem Cells / metabolism Neuronal Outgrowth Spinal Cord Injuries / metabolism Spinal Cord Injuries / physiopathology Spinal Cord Regeneration* Up-Regulation Zebrafish Zebrafish Proteins / genetics Zebrafish Proteins / metabolism* |
| IF | 4.931 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Peer-review(Publons) |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ゼブラフィッシュ | Tg(gfap:dTomato) |