論文 - 詳細
| RRC ID | 70785 |
|---|---|
| 著者 | Kula-Eversole E, Lee DH, Samba I, Yildirim E, Levine DC, Hong HK, Lear BC, Bass J, Rosbash M, Allada R. |
| タイトル | Phosphatase of Regenerating Liver-1 Selectively Times Circadian Behavior in Darkness via Function in PDF Neurons and Dephosphorylation of TIMELESS. |
| ジャーナル | Curr Biol |
| Abstract |
The timing of behavior under natural light-dark conditions is a function of circadian clocks and photic input pathways, but a mechanistic understanding of how these pathways collaborate in animals is lacking. Here we demonstrate in Drosophila that the Phosphatase of Regenerating Liver-1 (PRL-1) sets period length and behavioral phase gated by photic signals. PRL-1 knockdown in PDF clock neurons dramatically lengthens circadian period. PRL-1 mutants exhibit allele-specific interactions with the light- and clock-regulated gene timeless (tim). Moreover, we show that PRL-1 promotes TIM accumulation and dephosphorylation. Interestingly, the PRL-1 mutant period lengthening is suppressed in constant light, and PRL-1 mutants display a delayed phase under short, but not long, photoperiod conditions. Thus, our studies reveal that PRL-1-dependent dephosphorylation of TIM is a core mechanism of the clock that sets period length and phase in darkness, enabling the behavioral adjustment to change day-night cycles. |
| 巻・号 | 31(1) |
| ページ | 138-149.e5 |
| 公開日 | 2021-1-11 |
| DOI | 10.1016/j.cub.2020.10.013 |
| PII | S0960-9822(20)31517-7 |
| PMID | 33157022 |
| PMC | PMC7855481 |
| MeSH | Animals Animals, Genetically Modified Circadian Rhythm / physiology* Darkness Drosophila Proteins / genetics Drosophila Proteins / metabolism* Drosophila melanogaster Female Gene Knockdown Techniques Male Mutation Neurons / metabolism* Neuropeptides / metabolism Phosphorylation / physiology Photoperiod Protein Tyrosine Phosphatases / genetics Protein Tyrosine Phosphatases / metabolism* Time Factors |
| IF | 9.601 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 9 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | DGRC#142132 DGRC#112951 |