RRC ID 70785
著者 Kula-Eversole E, Lee DH, Samba I, Yildirim E, Levine DC, Hong HK, Lear BC, Bass J, Rosbash M, Allada R.
タイトル Phosphatase of Regenerating Liver-1 Selectively Times Circadian Behavior in Darkness via Function in PDF Neurons and Dephosphorylation of TIMELESS.
ジャーナル Curr Biol
Abstract The timing of behavior under natural light-dark conditions is a function of circadian clocks and photic input pathways, but a mechanistic understanding of how these pathways collaborate in animals is lacking. Here we demonstrate in Drosophila that the Phosphatase of Regenerating Liver-1 (PRL-1) sets period length and behavioral phase gated by photic signals. PRL-1 knockdown in PDF clock neurons dramatically lengthens circadian period. PRL-1 mutants exhibit allele-specific interactions with the light- and clock-regulated gene timeless (tim). Moreover, we show that PRL-1 promotes TIM accumulation and dephosphorylation. Interestingly, the PRL-1 mutant period lengthening is suppressed in constant light, and PRL-1 mutants display a delayed phase under short, but not long, photoperiod conditions. Thus, our studies reveal that PRL-1-dependent dephosphorylation of TIM is a core mechanism of the clock that sets period length and phase in darkness, enabling the behavioral adjustment to change day-night cycles.
巻・号 31(1)
ページ 138-149.e5
公開日 2021-1-11
DOI 10.1016/j.cub.2020.10.013
PII S0960-9822(20)31517-7
PMID 33157022
PMC PMC7855481
MeSH Animals Animals, Genetically Modified Circadian Rhythm / physiology* Darkness Drosophila Proteins / genetics Drosophila Proteins / metabolism* Drosophila melanogaster Female Gene Knockdown Techniques Male Mutation Neurons / metabolism* Neuropeptides / metabolism Phosphorylation / physiology Photoperiod Protein Tyrosine Phosphatases / genetics Protein Tyrosine Phosphatases / metabolism* Time Factors
IF 9.601
リソース情報
ショウジョウバエ DGRC#142132 DGRC#112951