RRC ID |
70785
|
著者 |
Kula-Eversole E, Lee DH, Samba I, Yildirim E, Levine DC, Hong HK, Lear BC, Bass J, Rosbash M, Allada R.
|
タイトル |
Phosphatase of Regenerating Liver-1 Selectively Times Circadian Behavior in Darkness via Function in PDF Neurons and Dephosphorylation of TIMELESS.
|
ジャーナル |
Curr Biol
|
Abstract |
The timing of behavior under natural light-dark conditions is a function of circadian clocks and photic input pathways, but a mechanistic understanding of how these pathways collaborate in animals is lacking. Here we demonstrate in Drosophila that the Phosphatase of Regenerating Liver-1 (PRL-1) sets period length and behavioral phase gated by photic signals. PRL-1 knockdown in PDF clock neurons dramatically lengthens circadian period. PRL-1 mutants exhibit allele-specific interactions with the light- and clock-regulated gene timeless (tim). Moreover, we show that PRL-1 promotes TIM accumulation and dephosphorylation. Interestingly, the PRL-1 mutant period lengthening is suppressed in constant light, and PRL-1 mutants display a delayed phase under short, but not long, photoperiod conditions. Thus, our studies reveal that PRL-1-dependent dephosphorylation of TIM is a core mechanism of the clock that sets period length and phase in darkness, enabling the behavioral adjustment to change day-night cycles.
|
巻・号 |
31(1)
|
ページ |
138-149.e5
|
公開日 |
2021-1-11
|
DOI |
10.1016/j.cub.2020.10.013
|
PII |
S0960-9822(20)31517-7
|
PMID |
33157022
|
PMC |
PMC7855481
|
MeSH |
Animals
Animals, Genetically Modified
Circadian Rhythm / physiology*
Darkness
Drosophila Proteins / genetics
Drosophila Proteins / metabolism*
Drosophila melanogaster
Female
Gene Knockdown Techniques
Male
Mutation
Neurons / metabolism*
Neuropeptides / metabolism
Phosphorylation / physiology
Photoperiod
Protein Tyrosine Phosphatases / genetics
Protein Tyrosine Phosphatases / metabolism*
Time Factors
|
IF |
9.601
|
リソース情報 |
ショウジョウバエ |
DGRC#142132
DGRC#112951 |