論文 - 詳細
| RRC ID | 71179 |
|---|---|
| 著者 | Pitt A, Schmidt J, Koll U, Hahn MW. |
| タイトル | Aquiluna borgnonia gen. nov., sp. nov., a member of a Microbacteriaceae lineage of freshwater bacteria with small genome sizes. |
| ジャーナル | Int J Syst Evol Microbiol |
| Abstract |
The actinobacterial strain 15G-AUS-rotT was isolated from an artificial pond located near Salzburg, Austria. The strain showed 16S rRNA gene sequence similarities of 98.7 % to Candidatus Aquiluna rubra and of 96.6 and 96.7 % to the two validly described species of the genus Rhodoluna. Phylogenetic reconstructions based on 16S rRNA gene sequences and genome-based on amino acid sequences of 118 single copy genes referred strain 15G-AUS-rotT to the family Microbacteriaceae and therein to the so-called subcluster Luna-1. The genome-based phylogenetic tree showed that the new strain represents a putative new genus. Cultures of strain 15G-AUS-rotT were light red pigmented and comprised very small, rod-shaped cells. They metabolized a broad variety of substrates. Major fatty acids (>10 %) of cells were iso-C16 : 0, antiso-C15 : 0 and iso-C14 : 0. The major respiratory quinone was MK-11 and a minor component was MK-10. The peptidoglycan structure belonged to an unusual B type. The closed genome sequence of the strain was very small (1.4 Mbp) and had a DNA G+C content of 54.8 mol%. An interesting feature was the presence of genes putatively encoding the complete light-driven proton pumping actinorhodopsin/retinal system, which were located at three different positions of the genome. Based on the characteristics of the strain, a new genus and a new species termed Aquiluna borgnonia is proposed for strain 15G-AUS-rotT (=DSM 107803T=JCM 32974T). |
| 巻・号 | 71(5) |
| 公開日 | 2021-5-1 |
| DOI | 10.1099/ijsem.0.004825 |
| PMID | 33999796 |
| MeSH | Actinobacteria / classification* Actinobacteria / isolation & purification Austria Bacterial Typing Techniques Base Composition Cell Wall / chemistry DNA, Bacterial / genetics Fatty Acids / chemistry Fresh Water / microbiology* Genome Size Peptidoglycan / chemistry Phylogeny* Pigmentation Ponds / microbiology RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Vitamin K 2 / chemistry |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 8 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM32974 |