論文 - 詳細
| RRC ID | 71255 |
|---|---|
| 著者 | Zakharyuk A, Valyshev A, Plotnikov A, Kopitsyn D, Suzina N, Shcherbakova V. |
| タイトル | Trichococcus shcherbakoviae subsp. psychrophilus subsp. nov., a psychrotolerant facultative anaerobe isolated from a cold spring. |
| ジャーナル | Int J Syst Evol Microbiol |
| Abstract |
A psychrotolerant facultative anaerobe, strain SKBGT, was isolated from the bottom sediments of the cold mineral spring Buxichen (Buryatia, Russia). Gram-positive non-motile cocci with a diameter of 1.75-2.5 µm were observed singly or in long chains. Cells grew in the temperature range from ̶ 5-35 °C. Growth was observed within the pH range of 7.0-9.5, with the optimum growth at pH 7.6 and at a NaCl concentration from 0-1.0 % (optimum 0.1 % (w/v)). Strain SKBGT was a chemoorganoheterotroph that used sugars and some organic acids as substrates. The predominant fatty acids in cell walls were С16:1ω9, С18:1ω9, and С16 : 0. The 16S rRNA gene sequence of strain SKBGT shared high similarity (>99 %) with those of the type strains of the genus Trichococcus. Digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between strain SKBGT and Trichococcus shcherbakoviae ArtT (=DSM 107162T=VKM B-3260T) were 70.1 and 95.4 %, respectively. The genomic DNA G+C content of strain SKBGT was 47.1 mol%. Compared with the type strain of T. shcherbakoviae, the new strain was characterized by a temperature optimum for growth (10 °C) significantly lower than that of T. shcherbakoviae DSM 107162T (20-30 °C). Based on phenotypic and genomic characteristics, the isolate SKBGT was classified as T. shcherbakoviae subsp. psychrophilus subsp. nov. The type strain is SKBGT (=VKM B-3241Т=JCM 33326T). |
| 巻・号 | 71(10) |
| 公開日 | 2021-10-1 |
| DOI | 10.1099/ijsem.0.005068 |
| PMID | 34694985 |
| MeSH | Bacterial Typing Techniques Base Composition Carnobacteriaceae / classification* Carnobacteriaceae / isolation & purification DNA, Bacterial / genetics Fatty Acids / chemistry Natural Springs / microbiology* Nucleic Acid Hybridization Phylogeny* RNA, Ribosomal, 16S / genetics Russia Sequence Analysis, DNA |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM33326 |