RRC ID 715
Author Ogihara Y, Yamazaki Y, Murai K, Kanno A, Terachi T, Shiina T, Miyashita N, Nasuda S, Nakamura C, Mori N, Takumi S, Murata M, Futo S, Tsunewaki K.
Title Structural dynamics of cereal mitochondrial genomes as revealed by complete nucleotide sequencing of the wheat mitochondrial genome.
Journal Nucleic Acids Res
Abstract The application of a new gene-based strategy for sequencing the wheat mitochondrial genome shows its structure to be a 452 528 bp circular molecule, and provides nucleotide-level evidence of intra-molecular recombination. Single, reciprocal and double recombinant products, and the nucleotide sequences of the repeats that mediate their formation have been identified. The genome has 55 genes with exons, including 35 protein-coding, 3 rRNA and 17 tRNA genes. Nucleotide sequences of seven wheat genes have been determined here for the first time. Nine genes have an exon-intron structure. Gene amplification responsible for the production of multicopy mitochondrial genes, in general, is species-specific, suggesting the recent origin of these genes. About 16, 17, 15, 3.0 and 0.2% of wheat mitochondrial DNA (mtDNA) may be of genic (including introns), open reading frame, repetitive sequence, chloroplast and retro-element origin, respectively. The gene order of the wheat mitochondrial gene map shows little synteny to the rice and maize maps, indicative that thorough gene shuffling occurred during speciation. Almost all unique mtDNA sequences of wheat, as compared with rice and maize mtDNAs, are redundant DNA. Features of the gene-based strategy are discussed, and a mechanistic model of mitochondrial gene amplification is proposed.
Volume 33(19)
Pages 6235-50
Published 2005-1-1
DOI 10.1093/nar/gki925
PII 33/19/6235
PMID 16260473
PMC PMC1275586
MeSH Base Sequence Chromosome Mapping DNA Shuffling DNA, Chloroplast / chemistry DNA, Mitochondrial / chemistry* Evolution, Molecular Genome, Plant* Mitochondria / genetics* Molecular Sequence Data Recombination, Genetic Sequence Alignment Sequence Analysis, DNA Triticum / genetics*
IF 11.502
Times Cited 148
Wheat CS