論文 - 詳細
| RRC ID | 7177 |
|---|---|
| 著者 | Doyle A, Martín-García R, Coulton AT, Bagley S, Mulvihill DP. |
| タイトル | Fission yeast Myo51 is a meiotic spindle pole body component with discrete roles during cell fusion and spore formation. |
| ジャーナル | J Cell Sci |
| Abstract |
Class V myosins are dimeric actin-associated motor proteins that deliver cellular cargoes to discrete cellular locations. Fission yeast possess two class V myosins, Myo51 and Myo52. Although Myo52 has been shown to have roles in vacuole distribution, cytokinesis and cell growth, Myo51 has no as yet discernible function in the vegetative life cycle. Here, we uncover distinct functions for this motor protein during mating and meiosis. Not only does Myo51 transiently localise to a foci at the site of cell fusion upon conjugation, but overexpression of the Myo51 globular tail also leads to disruption of cell fusion. Upon completion of meiotic prophase Myo51 localises to the outside of the spindle pole bodies (SPBs), where it remains until completion of meiosis II. Association of Myo51 with SPBs is not dependent upon actin or the septation initiation network (SIN); however, it is dependent on a stable microtubule cytoskeleton and the presence of the Cdc2-CyclinB complex. We observe a rapid and dynamic exchange of Myo51 at the SPB during meiosis I but not meiosis II. Finally, we show that Myo51 has an important role in regulating spore formation upon completion of meiosis. |
| 巻・号 | 122(Pt 23) |
| ページ | 4330-40 |
| 公開日 | 2009-12-1 |
| DOI | 10.1242/jcs.055202 |
| PII | jcs.055202 |
| PMID | 19887589 |
| MeSH | Cell Cycle / genetics Cell Cycle / physiology* Meiosis / genetics Meiosis / physiology Microscopy, Fluorescence Myosin Type V / genetics Myosin Type V / metabolism Myosin Type V / physiology* Schizosaccharomyces / metabolism* Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism Schizosaccharomyces pombe Proteins / physiology* Spindle Apparatus / metabolism* Spores, Fungal / growth & development* Spores, Fungal / metabolism |
| IF | 4.573 |
| 引用数 | 20 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | F1000Research |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 酵母 | strains? |