論文 - 詳細
| RRC ID | 71871 |
|---|---|
| 著者 | Ko JA, Yanai R, Nishida T. |
| タイトル | Up-regulation of ZO-1 expression and barrier function in cultured human corneal epithelial cells by substance P. |
| ジャーナル | FEBS Lett |
| Abstract |
The effects of the sensory neurotransmitter substance P on the expression of tight junction proteins and on barrier function in human corneal epithelial cells were investigated. The expression of ZO-1, but not that of occludin or claudin-1, was increased by substance P in a concentration- and time-dependent manner. This effect was inhibited by the NK-1 receptor antagonist GR82334 and by KN62, an inhibitor of Ca(2+)- and calmodulin-dependent protein kinase II. Substance P also increased the transepithelial electrical resistance of a cell monolayer in a manner sensitive to GR82334. Substance P may therefore play a role in maintenance of tight junctions in the corneal epithelium. |
| 巻・号 | 583(12) |
| ページ | 2148-53 |
| 公開日 | 2009-6-18 |
| DOI | 10.1016/j.febslet.2009.05.010 |
| PII | S0014-5793(09)00380-9 |
| PMID | 19446555 |
| MeSH | 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine / analogs & derivatives 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine / pharmacology Base Sequence Calcium-Calmodulin-Dependent Protein Kinase Type 2 / antagonists & inhibitors Cells, Cultured Claudin-1 DNA Primers / genetics Electric Impedance Epithelium, Corneal / cytology Epithelium, Corneal / drug effects* Epithelium, Corneal / metabolism* Humans Membrane Proteins / genetics* Membrane Proteins / metabolism* Neurokinin-1 Receptor Antagonists Occludin Phosphoproteins / genetics* Phosphoproteins / metabolism* Physalaemin / analogs & derivatives Physalaemin / pharmacology Substance P / metabolism Substance P / pharmacology* Tight Junctions / drug effects Tight Junctions / metabolism Up-Regulation / drug effects Zonula Occludens-1 Protein |
| IF | 3.057 |
| リソース情報 | |
| ヒト・動物細胞 | HCE-T(RCB2280) |