RRC ID 71899
Author Chinen T, Yamazaki K, Hashimoto K, Fujii K, Watanabe K, Takeda Y, Yamamoto S, Nozaki Y, Tsuchiya Y, Takao D, Kitagawa D.
Title Centriole and PCM cooperatively recruit CEP192 to spindle poles to promote bipolar spindle assembly.
Journal J Cell Biol
Abstract The pericentriolar material (PCM) that accumulates around the centriole expands during mitosis and nucleates microtubules. Here, we show the cooperative roles of the centriole and PCM scaffold proteins, pericentrin and CDK5RAP2, in the recruitment of CEP192 to spindle poles during mitosis. Systematic depletion of PCM proteins revealed that CEP192, but not pericentrin and/or CDK5RAP2, was crucial for bipolar spindle assembly in HeLa, RPE1, and A549 cells with centrioles. Upon double depletion of pericentrin and CDK5RAP2, CEP192 that remained at centriole walls was sufficient for bipolar spindle formation. In contrast, through centriole removal, we found that pericentrin and CDK5RAP2 recruited CEP192 at the acentriolar spindle pole and facilitated bipolar spindle formation in mitotic cells with one centrosome. Furthermore, the perturbation of PLK1, a critical kinase for PCM assembly, efficiently suppressed bipolar spindle formation in mitotic cells with one centrosome. Overall, these data suggest that the centriole and PCM scaffold proteins cooperatively recruit CEP192 to spindle poles and facilitate bipolar spindle formation.
Volume 220(2)
Published 2021-2-1
DOI 10.1083/jcb.202006085
PII 211683
PMID 33443571
PMC PMC7812875
MeSH Antigens / metabolism Cell Cycle Proteins / metabolism Centrioles / drug effects Centrioles / metabolism* Chromosomal Proteins, Non-Histone / metabolism* HeLa Cells Humans Mitosis / drug effects Models, Biological Nerve Tissue Proteins / metabolism Protein Serine-Threonine Kinases / metabolism Proto-Oncogene Proteins / metabolism Pyrimidines / pharmacology Spindle Poles / drug effects Spindle Poles / metabolism* Sulfones / pharmacology
IF 8.811
Human and Animal Cells A549 DU145(RCB2143) PC-3(RCB2145) PANC-1(RCB2145) GI-1(RCB0763) A431 MCF7(RCB1904)