RRC ID 71988
著者 Mito T, Seki M, Shinozaki K, Ohme-Takagi M, Matsui K.
タイトル Generation of chimeric repressors that confer salt tolerance in Arabidopsis and rice.
ジャーナル Plant Biotechnol J
Abstract We show here that transgenic Arabidopsis plants that expressed chimeric repressors derived from the AtMYB102, ANAC047, HRS1, ZAT6 and AtERF5 transcription factors were tolerant to treatment with 400 mm NaCl, which was lethal to wild-type plants. The transgenic plants grew well, without any apparent differences from the wild-type plants under normal growth condition. The transgenic lines expressing the AtMYB102, ANAC047 and HRS1 chimeric repressors germinated in the presence of 225 mm NaCl, while those expressing the ZAT6 and AtERF5 did not. However, the latter lines were tolerant to osmotic stress and germinated in the presence of 600 mm mannitol, suggesting a link between responses to salt and osmotic stress. Expression of the AtMYB102, ANAC047, ZAT6 and AtERF5 genes was induced by salt treatment, while that of HRS1 was repressed. HRS1 has transcriptional repressive activity and appears to suppress the expression of factors that negatively regulate salt tolerance. Microarray analysis revealed that the levels of expression of DREB1A, DREB2B and several genes for ZAT transcription factors rose 10- to 100-fold in the AtMYB102 chimeric repressor line under both normal and stress conditions. Elevated expression of DREB- and ZAT- related genes might be involved in the salt tolerance of the AtMYB102 chimeric repressor line. Transgenic rice plants expressing chimeric repressors derived from Os02g0325600 and Os03g0327800, rice homologues of HRS1 and ANAC047, were tolerant to salinity stress demonstrated by suppression of growth inhibition and ion leakages. Expression of a chimeric repressor provides an effective strategy for enhancing tolerance of plants to abiotic stress.
巻・号 9(7)
ページ 736-46
公開日 2011-9-1
DOI 10.1111/j.1467-7652.2010.00578.x
PMID 21114612
MeSH Arabidopsis / drug effects Arabidopsis / genetics* Arabidopsis / metabolism Arabidopsis / physiology Gene Expression Profiling Gene Expression Regulation, Plant / drug effects Gene Library Mannitol / pharmacology Oligonucleotide Array Sequence Analysis Oryza / drug effects Oryza / genetics* Oryza / metabolism Oryza / physiology Osmotic Pressure / physiology Plant Proteins / genetics Plant Proteins / metabolism Plant Roots / genetics Plant Roots / metabolism Plant Roots / physiology Plant Shoots / genetics Plant Shoots / metabolism Plant Shoots / physiology Plants, Genetically Modified / drug effects Plants, Genetically Modified / genetics Plants, Genetically Modified / metabolism Plants, Genetically Modified / physiology RNA, Plant / genetics Salt Tolerance* / physiology Seedlings / genetics Seedlings / metabolism Seedlings / physiology Sodium Chloride / pharmacology Stress, Physiological Transcription Factors / genetics Transcription Factors / metabolism* Water / metabolism
IF 8.154
リソース情報
シロイヌナズナ / 植物培養細胞・遺伝子 pda05157 pda18125 pda01657 pda11897 pda00826