論文 - 詳細
| RRC ID | 72436 |
|---|---|
| 著者 | Han SK, Jeon SJ, Miyazawa K, Yi SY, Yoo YS. |
| タイトル | Enhancement of anti-inflammatory tendency by SB203580, p38alpha specific inhibitor, in human fibroblast-like synoviocyte cell line, MH7A. |
| ジャーナル | Rheumatol Int |
| Abstract |
Interleukin-1 beta (IL-1beta) is an abundant cytokine, which, together with TNF-alpha, mediates inflammatory events in rheumatoid arthritis (RA). IL-1beta is known to induce the induction of inflammatory cytokines and metalloproteinases (MMPs) in rheumatoid synovial cells. Here, we assessed these inflammatory events by measuring IL-1beta levels in the human synovial cell line, MH7A. We observed that the activation of p38 MAP kinase by IL-1beta was involved in the induction of inflammatory cytokines, as well as several genes, including MMP-1 and MMP-3. SB203580, a specific p38 MAP kinase inhibitor, inhibited the production of IL-1beta-induced cytokines and MMPs, while the levels of the tissue inhibitor of metalloproteinase (TIMPs) were unchanged by treatment with SB203580. Moreover, the induction of suppressor of cytokine signaling 3 (SOCS3) and interferon regulatory factor 1 (IRF-1) were both found to be induced by the inhibition of p38 MAP kinase. Therefore, we suggested that the inhibition of p38 MAP kinase might enhance anti-inflammatory tendencies in the MH7A cells. |
| 巻・号 | 26(11) |
| ページ | 972-8 |
| 公開日 | 2006-9-1 |
| DOI | 10.1007/s00296-006-0109-4 |
| PMID | 16532349 |
| MeSH | Anti-Inflammatory Agents, Non-Steroidal / pharmacology* Arthritis, Rheumatoid / metabolism Cell Line Down-Regulation Enzyme Inhibitors / pharmacology* Fibroblasts / enzymology* Fibroblasts / metabolism Humans Imidazoles / pharmacology* Interleukin-1beta / metabolism Interleukin-6 / biosynthesis Interleukin-6 / genetics Matrix Metalloproteinase 3 / genetics Matrix Metalloproteinase 3 / metabolism Mitogen-Activated Protein Kinase 14 / antagonists & inhibitors* Phosphorylation Promoter Regions, Genetic Pyridines / pharmacology* RNA, Messenger / metabolism Suppressor of Cytokine Signaling 3 Protein Suppressor of Cytokine Signaling Proteins / genetics Suppressor of Cytokine Signaling Proteins / metabolism Synovial Membrane / enzymology* |
| IF | 1.984 |
| リソース情報 | |
| ヒト・動物細胞 | MH7A(RCB1512) |