論文 - 詳細
| RRC ID | 72697 |
|---|---|
| 著者 | Kawami M, Takenaka S, Akai M, Yumoto R, Takano M. |
| タイトル | Characterization of miR-34a-Induced Epithelial-Mesenchymal Transition in Non-Small Lung Cancer Cells Focusing on p53. |
| ジャーナル | Biomolecules |
| Abstract |
BACKGROUND:Epithelial-mesenchymal transition (EMT), a phenotypic conversion of the epithelial to mesenchymal state, contributes to cancer progression. Currently, several microRNAs (miRNAs) are associated with EMT-mediated cancer progression, but the contribution of miR-34a to EMT in cancer cells remains controversial. The present study aimed to clarify the role of miR-34a in the EMT-related phenotypes of human non-small cell lung cancer (NSCLC) cell lines, A549 (p53 wild-type) and H1299 (p53-deficient). METHODS:The miR-34a mimic and p53 small interfering RNA (siRNA) were transfected into the cells using Lipofectamine, and the obtained total RNA and cell lysates were used for real-time polymerase chain reaction and Western blotting analysis, respectively. RESULTS:The introduction of the miR-34a mimic led to an increase in the mRNA and protein expression levels of α-smooth muscle actin (α-SMA), a mesenchymal marker gene, in A549, but not in H1299 cells. Additionally, miR-34a-induced the upregulation of p53 activity and migration was observed in A549, but not in H1299 cells. However, under the p53-knockdown condition, only α-SMA upregulation by miR-34a was abolished. CONCLUSION:These findings indicate a close relationship between p53 and miR-34a-induced EMT in p53-wild type NSCLC cells, which provides novel insights about the role of miR-34a in EMT-like phenotypic changes in NSCLC. |
| 巻・号 | 11(12) |
| 公開日 | 2021-12-9 |
| DOI | 10.3390/biom11121853 |
| PII | biom11121853 |
| PMID | 34944497 |
| PMC | PMC8699678 |
| MeSH | A549 Cells Actins / metabolism Carcinoma, Non-Small-Cell Lung / genetics* Carcinoma, Non-Small-Cell Lung / metabolism Cell Line, Tumor Epithelial-Mesenchymal Transition Gene Expression Profiling Gene Expression Regulation, Neoplastic Humans Lung Neoplasms / genetics* Lung Neoplasms / metabolism MicroRNAs / genetics* Tumor Suppressor Protein p53 / genetics* Up-Regulation |
| IF | 4.082 |
| リソース情報 | |
| ヒト・動物細胞 | A549 |