| RRC ID |
72956
|
| 著者 |
Katada K, Naito Y, Shimozawa M, Mizushima K, Kuroda M, Takagi T, Kokura S, Ichikawa H, Yoshida N, Matsui H, Yoshikawa T.
|
| タイトル |
Gene expression analysis following hypoxia-reoxygenation in rat gastric epithelial cells using a high-density oligonucleotide array.
|
| ジャーナル |
Redox Rep
|
| Abstract |
Recent investigations have demonstrated that the signaling of hypoxia-re-oxygenation is a major contributing pathway leading to gastric mucosal injury induced by stress, non-steroidal anti-inflammatory drugs, and Helicobacter pylori. The aim of the present study was to perform a gene expression analysis on the gastric mucosal cellular response to hypoxia-reoxygenation using a high-density oligonucleotide array. Cells were subjected to hypoxia with 95% N(2) and 5% CO(2) at 37 degrees C for 2 h. Reoxygenation was initiated by placing the cells in an environment of normoxia for 2 h. Total RNA was extracted, and differences in gene expression profiles between the normoxia and hypoxia-reoxygenation groups were investigated using a GeneChip of Rat Toxicology U34 array (Affymetrix). Hypoxia-reoxygenation up-regulated the stress-related genes (heat shock protein-70 [HSP-70], catalase). The enhanced expression of HSP-70 was confirmed by Western blot analysis. In conclusion, these results suggest that up-regulation of the HSP-70 gene after reoxygenation may play a role in maintaining cell survival and supporting cell function as a molecular chaperone.
|
| 巻・号 |
9(6)
|
| ページ |
337-42
|
| 公開日 |
2004-1-1
|
| DOI |
10.1179/135100004225006849
|
| PMID |
15720829
|
| MeSH |
Animals
Catalase / genetics
Cell Hypoxia* / genetics
Cell Hypoxia* / physiology
Cell Line
Down-Regulation
Epithelial Cells / metabolism*
Gastric Mucosa / cytology
Gastric Mucosa / metabolism*
Gene Expression Profiling
Gene Expression Regulation*
HSP70 Heat-Shock Proteins / genetics
Heat-Shock Proteins / genetics*
Oligonucleotide Array Sequence Analysis
Rats
Up-Regulation
|
| IF |
2.753
|
| リソース情報 |
| ヒト・動物細胞 |
RGM1(RCB0876) |
