RRC ID 72956
Author Katada K, Naito Y, Shimozawa M, Mizushima K, Kuroda M, Takagi T, Kokura S, Ichikawa H, Yoshida N, Matsui H, Yoshikawa T.
Title Gene expression analysis following hypoxia-reoxygenation in rat gastric epithelial cells using a high-density oligonucleotide array.
Journal Redox Rep
Abstract Recent investigations have demonstrated that the signaling of hypoxia-re-oxygenation is a major contributing pathway leading to gastric mucosal injury induced by stress, non-steroidal anti-inflammatory drugs, and Helicobacter pylori. The aim of the present study was to perform a gene expression analysis on the gastric mucosal cellular response to hypoxia-reoxygenation using a high-density oligonucleotide array. Cells were subjected to hypoxia with 95% N(2) and 5% CO(2) at 37 degrees C for 2 h. Reoxygenation was initiated by placing the cells in an environment of normoxia for 2 h. Total RNA was extracted, and differences in gene expression profiles between the normoxia and hypoxia-reoxygenation groups were investigated using a GeneChip of Rat Toxicology U34 array (Affymetrix). Hypoxia-reoxygenation up-regulated the stress-related genes (heat shock protein-70 [HSP-70], catalase). The enhanced expression of HSP-70 was confirmed by Western blot analysis. In conclusion, these results suggest that up-regulation of the HSP-70 gene after reoxygenation may play a role in maintaining cell survival and supporting cell function as a molecular chaperone.
Volume 9(6)
Pages 337-42
Published 2004-1-1
DOI 10.1179/135100004225006849
PMID 15720829
MeSH Animals Catalase / genetics Cell Hypoxia* / genetics Cell Hypoxia* / physiology Cell Line Down-Regulation Epithelial Cells / metabolism* Gastric Mucosa / cytology Gastric Mucosa / metabolism* Gene Expression Profiling Gene Expression Regulation* HSP70 Heat-Shock Proteins / genetics Heat-Shock Proteins / genetics* Oligonucleotide Array Sequence Analysis Rats Up-Regulation
IF 2.753
Human and Animal Cells RGM1(RCB0876)