RRC ID 73021
Author Xiang Y, Nakabayashi K, Ding J, He F, Bentsink L, Soppe WJ.
Title Reduced Dormancy5 encodes a protein phosphatase 2C that is required for seed dormancy in Arabidopsis.
Journal Plant Cell
Abstract Seed dormancy determines germination timing and contributes to crop production and the adaptation of natural populations to their environment. Our knowledge about its regulation is limited. In a mutagenesis screen of a highly dormant Arabidopsis thaliana line, the reduced dormancy5 (rdo5) mutant was isolated based on its strongly reduced seed dormancy. Cloning of RDO5 showed that it encodes a PP2C phosphatase. Several PP2C phosphatases belonging to clade A are involved in abscisic acid signaling and control seed dormancy. However, RDO5 does not cluster with clade A phosphatases, and abscisic acid levels and sensitivity are unaltered in the rdo5 mutant. RDO5 transcript could only be detected in seeds and was most abundant in dry seeds. RDO5 was found in cells throughout the embryo and is located in the nucleus. A transcriptome analysis revealed that several genes belonging to the conserved PUF family of RNA binding proteins, in particular Arabidopsis PUMILIO9 (APUM9) and APUM11, showed strongly enhanced transcript levels in rdo5 during seed imbibition. Further transgenic analyses indicated that APUM9 reduces seed dormancy. Interestingly, reduction of APUM transcripts by RNA interference complemented the reduced dormancy phenotype of rdo5, indicating that RDO5 functions by suppressing APUM transcript levels.
Volume 26(11)
Pages 4362-75
Published 2014-11-1
DOI 10.1105/tpc.114.132811
PII tpc.114.132811
PMID 25415980
PMC PMC4277229
MeSH Abscisic Acid / metabolism* Arabidopsis / enzymology* Arabidopsis / genetics Arabidopsis / physiology Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* Gene Expression Profiling Gene Expression Regulation, Plant* Germination Mutation Phenotype Phosphoprotein Phosphatases / genetics Phosphoprotein Phosphatases / metabolism* Plant Dormancy Plant Growth Regulators / metabolism* Plants, Genetically Modified Protein Phosphatase 2C RNA-Binding Proteins / genetics RNA-Binding Proteins / metabolism* Seeds / enzymology Seeds / genetics Seeds / physiology Signal Transduction*
IF 9.618
Resource
Arabidopsis / Cultured plant cells, genes pst18302