RRC ID 73048
Author Hong J, Ishihara K, Yamaki K, Hiraizumi K, Ohno T, Ahn JW, Zee O, Ohuchi K.
Title Apicidin, a histone deacetylase inhibitor, induces differentiation of HL-60 cells.
Journal Cancer Lett
Abstract The fungal metabolite apicidin (cyclo(N-O-methyl-L-tryptophanyl-L-isoleucinyl-D-pipecolinyl-L-2-amino-8-oxodecanoyl)) inhibited the growth of HL-60 cells in a concentration-dependent manner (100-1000 nM). At higher concentrations (>300 nM), cell death was induced. At 100 nM, it induced hyperacetylation of histone H4 time-dependently, while trichostatin A induced transient hyperacetylation. Apicidin (10-100 nM) increased the cells having nitroblue tetrazolium-reducing activity and expressing CD11b but not CD14 and CD15. The expression of CD11b by apicidin was long lasting, while that by trichostatin A was transient. In K562 cells, apicidin at 10-100 nM did not inhibit cell growth nor express CD11b, CD14 and CD15. Our findings indicate that apicidin inhibits proliferation and induces the early stage of differentiation of HL-60 cells.
Volume 189(2)
Pages 197-206
Published 2003-1-28
DOI 10.1016/s0304-3835(02)00500-1
PII S0304383502005001
PMID 12490313
MeSH Acetylation / drug effects Apoptosis / drug effects CD11b Antigen / analysis Cell Differentiation / drug effects Dose-Response Relationship, Drug Enzyme Inhibitors / pharmacology* HL-60 Cells / cytology HL-60 Cells / drug effects* Histone Deacetylase Inhibitors* Histones / metabolism Humans K562 Cells / drug effects Lewis X Antigen / analysis Lipopolysaccharide Receptors / analysis Neoplasm Proteins / antagonists & inhibitors* Neoplasm Proteins / metabolism Peptides, Cyclic / pharmacology* Protein Processing, Post-Translational / drug effects
IF 7.36
Human and Animal Cells HL60(RCB0041)