論文 - 詳細
| RRC ID | 73065 |
|---|---|
| 著者 | Jawhari AU, Buda A, Jenkins M, Shehzad K, Sarraf C, Noda M, Farthing MJ, Pignatelli M, Adams JC. |
| タイトル | Fascin, an actin-bundling protein, modulates colonic epithelial cell invasiveness and differentiation in vitro. |
| ジャーナル | Am J Pathol |
| Abstract |
In epithelial tissue, cell-matrix and cell-cell adhesive interactions have important roles in the normal organization and stabilization of the cell layer. The malignant conversion of epithelial cells involves alterations in the expression and function of these adhesion systems that enable a switch to a migratory phenotype in tumor invasion and metastasis. Fascin is an actin-crosslinking protein that is found in the core actin bundles of cell-surface spikes and projections that are implicated in cell motility. We demonstrate that fascin is not detectable in normal colonic epithelium, but is dramatically up-regulated in colorectal adenocarcinoma. To test the hypothesis that fascin could participate in tumor invasive behavior, we developed a cell culture model to examine the effect of fascin expression on the adhesive interactions, invasiveness, and differentiation of colonic epithelial cells. We report marked effects on the organization of cell-surface protrusions, actin cytoskeleton, and focal adhesions in the absence of alterations in the protein levels of the major components of these structures. These effects correlate with alterations in cell movements on two-dimensional matrix, and increased invasiveness in three-dimensional matrix. The cells also show increased proliferation and decreased capacity for normal glandular differentiation in collagen gels. We propose that up-regulation of fascin, by promoting the formation of protrusive, actin-based, cell-motility structures, could be a significant component in the acquisition of invasive phenotype in colonic carcinoma. |
| 巻・号 | 162(1) |
| ページ | 69-80 |
| 公開日 | 2003-1-1 |
| DOI | 10.1016/S0002-9440(10)63799-6 |
| PII | S0002-9440(10)63799-6 |
| PMID | 12507891 |
| PMC | PMC1851132 |
| MeSH | Actins / metabolism Adenocarcinoma / metabolism* Adenocarcinoma / pathology Cadherins / metabolism Carrier Proteins / genetics Carrier Proteins / metabolism* Cell Differentiation / genetics Cell Division Cell Movement / genetics Cells, Cultured Collagen Type I / metabolism Collagen Type IV / metabolism Colon / cytology Colonic Neoplasms / metabolism* Colonic Neoplasms / pathology Cytoskeletal Proteins / metabolism Epithelial Cells* / cytology Epithelial Cells* / physiology Gene Expression Humans Integrin beta1 / metabolism Intestinal Mucosa / cytology Intestinal Mucosa / metabolism Laminin / metabolism Microfilament Proteins / genetics Microfilament Proteins / metabolism* Neoplasm Invasiveness / genetics Transfection Tumor Cells, Cultured Up-Regulation |
| IF | 3.491 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MKN7(RCB0999) MKN45(RCB1001) |