| RRC ID |
73340
|
| 著者 |
Andric V, Nevers A, Hazra D, Auxilien S, Menant A, Graille M, Palancade B, Rougemaille M.
|
| タイトル |
A scaffold lncRNA shapes the mitosis to meiosis switch.
|
| ジャーナル |
Nat Commun
|
| Abstract |
Long non-coding RNAs (lncRNAs) contribute to the regulation of gene expression in response to intra- or extracellular signals but the underlying molecular mechanisms remain largely unexplored. Here, we identify an uncharacterized lncRNA as a central player in shaping the meiotic gene expression program in fission yeast. We report that this regulatory RNA, termed mamRNA, scaffolds the antagonistic RNA-binding proteins Mmi1 and Mei2 to ensure their reciprocal inhibition and fine tune meiotic mRNA degradation during mitotic growth. Mechanistically, mamRNA allows Mmi1 to target Mei2 for ubiquitin-mediated downregulation, and conversely enables accumulating Mei2 to impede Mmi1 activity, thereby reinforcing the mitosis to meiosis switch. These regulations also occur within a unique Mmi1-containing nuclear body, positioning mamRNA as a spatially-confined sensor of Mei2 levels. Our results thus provide a mechanistic basis for the mutual control of gametogenesis effectors and further expand our vision of the regulatory potential of lncRNAs.
|
| 巻・号 |
12(1)
|
| ページ |
770
|
| 公開日 |
2021-2-3
|
| DOI |
10.1038/s41467-021-21032-7
|
| PII |
10.1038/s41467-021-21032-7
|
| PMID |
33536434
|
| PMC |
PMC7859202
|
| MeSH |
Gene Expression Regulation, Fungal*
Meiosis / genetics*
Mitosis / genetics*
Protein Binding
RNA Stability / genetics
RNA, Fungal / genetics*
RNA, Long Noncoding / genetics*
RNA, Messenger / genetics
RNA, Messenger / metabolism
RNA-Binding Proteins / genetics
RNA-Binding Proteins / metabolism
Schizosaccharomyces / genetics*
Schizosaccharomyces / metabolism
Schizosaccharomyces pombe Proteins / genetics
Schizosaccharomyces pombe Proteins / metabolism
mRNA Cleavage and Polyadenylation Factors / genetics
mRNA Cleavage and Polyadenylation Factors / metabolism
|
| IF |
12.121
|
| リソース情報 |
| 酵母 |
FYP3968 |
