Reference - Detail
| RRC ID | 73746 |
|---|---|
| Author | Tomosugi W, Matsuda T, Tani T, Nemoto T, Kotera I, Saito K, Horikawa K, Nagai T. |
| Title | An ultramarine fluorescent protein with increased photostability and pH insensitivity. |
| Journal | Nat Methods |
| Abstract |
We report a pH-insensitive and photostable ultramarine fluorescent protein, Sirius, with an emission peak at 424 nm, the shortest emission wavelength among fluorescent proteins reported to date. The pH-insensitivity of Sirius allowed prolonged visualization of biological events in an acidic environment. Two fluorescence resonance energy transfer (FRET) pairs, Sirius-mseCFP and Sapphire-DsRed, allowed dual-FRET imaging with single-wavelength excitation, enabling detection of Ca(2+) concentration and caspase-3 activation in the same apoptotic cells. |
| Volume | 6(5) |
| Pages | 351-3 |
| Published | 2009-5-1 |
| DOI | 10.1038/nmeth.1317 |
| PII | nmeth.1317 |
| PMID | 19349978 |
| MeSH | Amino Acid Substitution Animals Apoptosis Calcium Signaling Caspase 3 / metabolism Color Dictyostelium / microbiology Escherichia coli / genetics Escherichia coli / metabolism Fluorescence Resonance Energy Transfer Green Fluorescent Proteins / chemistry Green Fluorescent Proteins / genetics HeLa Cells Humans Hydrogen-Ion Concentration Luminescent Proteins / chemistry* Luminescent Proteins / genetics Molecular Sequence Data Mutagenesis Phagocytosis Photobleaching Protein Stability Recombinant Fusion Proteins / chemistry Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Spectrometry, Fluorescence Spectrophotometry, Ultraviolet Transfection |
| IF | 30.822 |
| Resource | |
| DNA material | Sirius/pcDNA3 (RDB19875) |