論文 - 詳細
| RRC ID | 73760 |
|---|---|
| 著者 | Arai Y, Takauchi H, Ogami Y, Fujiwara S, Nakano M, Matsuda T, Nagai T. |
| タイトル | Spontaneously Blinking Fluorescent Protein for Simple Single Laser Super-Resolution Live Cell Imaging. |
| ジャーナル | ACS Chem Biol |
| Abstract |
Super-resolution imaging techniques based on single molecule localization microscopy (SMLM) broke the diffraction limit of optical microscopy in living samples with the aid of photoswitchable fluorescent probes and intricate microscopy systems. Here, we developed a fluorescent protein, SPOON, which can be switched off by excitation light illumination and switched on by thermally induced dehydration, resulting in an apparent spontaneous blinking behavior. This unique property of SPOON provides a simple SMLM-based super-resolution imaging platform which requires only a single 488 nm laser. |
| 巻・号 | 13(8) |
| ページ | 1938-1943 |
| 公開日 | 2018-8-17 |
| DOI | 10.1021/acschembio.8b00200 |
| PMID | 29963852 |
| MeSH | Escherichia coli Fluorescence Fluorescent Dyes / chemistry* Fluorescent Dyes / radiation effects HeLa Cells Heating Humans Lasers Light Luminescent Proteins / chemistry* Luminescent Proteins / genetics Luminescent Proteins / radiation effects Microscopy, Fluorescence / instrumentation Microscopy, Fluorescence / methods Mutagenesis |
| IF | 4.434 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 38 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | SPOON/pcDNA3 (RDB19863) |