RRC ID 75
著者 Yamate J, Yasui H, Benn SJ, Tsukamoto Y, Kuwamura M, Kumagai D, Sakuma S, LaMarre J.
タイトル Characterization of newly established tumor lines from a spontaneous malignant schwannoma in F344 rats: nerve growth factor production, growth inhibition by transforming growth factor-beta1, and macrophage-like phenotype expression.
ジャーナル Acta Neuropathol
Abstract Transplantable tumor (KE) and clone cell (KE-F11) lines were established from a spontaneous malignant schwannoma found in an aged F344 rat. The primary tumor and KE tumors consisted of oval or spindle cells arranged in ill-defined bundles. Cultured KE-F11 cells exhibited polygonal or spindle configurations. Immunohistochemically, neoplastic cells in KE and KE-F11 reacted to vimentin, S-100 protein, neuron-specific enolase, myelin basic protein, and glial fibrillary acidic protein in varying degrees, indicating neurogenic features; occasional cells reacted to alpha-smooth muscle actin. Cells positive for lysosomal enzymes (acid phosphatase and non-specific esterase), and ED1 (rat macrophage specific) were observed in KE-F11, and electron microscopically, cells with many lysosomes were frequently present, indicating expression of macrophage-like phenotypes. Bioassay analysis revealed that KE-F11 cells produced high levels of nerve growth factor. DNA synthesis was inhibited by addition of transforming growth factor-beta1 (TGF-beta1), and Northern blot analysis revealed that expression of c-myc, a cell cycle-related immediate early gene, was depressed by TGF-beta1. Likely, TGF-beta1 is a factor capable of inhibiting cellular growth of Schwann cells. mRNA expression of the low-density lipoprotein receptor-related protein (LRP) was seen in KE-F11 cells by Northern blot analysis, and the level was decreased by lipopolysaccharide (LPS) treatment. LRP may be attributable to regulation of Schwann cell functions. KE-F11 cells seeded on laminin-coated dishes exhibited more extended cytoplasmic projections than on collagen type I-coated dishes. The present study provides evidence that biological properties of malignant schwannoma-derived cells might be affected by exogenous factors such as TGF-beta1, LPS and laminin. These tumor lines may be useful for studies on pathobiological characteristics of Schwann cells.
巻・号 106(3)
ページ 221-33
公開日 2003-9-1
DOI 10.1007/s00401-003-0723-0
PMID 12811582
MeSH Actins / metabolism Animals Blotting, Northern Cell Count Cell Line, Transformed Desmin / metabolism Dose-Response Relationship, Drug Genes, jun / physiology Genes, myc / physiology Glial Fibrillary Acidic Protein / metabolism In Vitro Techniques Keratins / metabolism Lipopolysaccharides / pharmacology Macrophages / pathology Macrophages / ultrastructure* Male Microscopy, Electron Myelin Basic Protein / metabolism Nerve Growth Factor / metabolism* Neurilemmoma / metabolism* Neurilemmoma / pathology Neurilemmoma / ultrastructure PC12 Cells Phenotype Phosphopyruvate Hydratase / metabolism RNA, Messenger / biosynthesis Rats Rats, Inbred F344 Reverse Transcriptase Polymerase Chain Reaction / methods S100 Proteins / metabolism Staining and Labeling Time Factors Transforming Growth Factor beta / metabolism* Transforming Growth Factor beta1 Vimentin / metabolism
IF 14.256
引用数 10
WOS 分野 PATHOLOGY CLINICAL NEUROLOGY NEUROSCIENCES
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